Detailed information of TA system TA154201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1328179..1329095
• Replicon: chromosome
• Accession: NZ_CP051466
• Organism: Bacillus subtilis subsp. subtilis strain UCMB5021

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: HC658_RS06945
• Protein ID: WP_003244695.1
• Coordinates: 1328349..1329095 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: HC658_RS06940
• Protein ID: WP_003232646.1
• Coordinates: 1328179..1328349 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC658_RS06905	1325042..1325371	+	330	WP_003232660.1	phage-like element PBSX protein XkdW	-
HC658_RS06910	1325368..1325532	+	165	WP_003232658.1	XkdX family protein	-
HC658_RS06915	1325576..1326415	+	840	WP_032721388.1	phage-like element PBSX protein XepA	-
HC658_RS06920	1326468..1326737	+	270	WP_003232655.1	hemolysin XhlA family protein	-
HC658_RS06925	1326750..1327013	+	264	WP_003232653.1	phage holin	-
HC658_RS06930	1327026..1327919	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
HC658_RS06935	1327956..1328093	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
HC658_RS06940	1328179..1328349	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
HC658_RS06945	1328349..1329095	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
HC658_RS06950	1329205..1330206	-	1002	WP_003232642.1	inorganic phosphate transporter	-
HC658_RS06955	1330219..1330836	-	618	WP_003218470.1	DUF47 domain-containing protein	-
HC658_RS06960	1331112..1332428	-	1317	WP_015252262.1	serine/threonine exchanger	-
HC658_RS06965	1332816..1333766	+	951	WP_106610746.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T154201 WP_003244695.1 NZ_CP051466:c1329095-1328349 [Bacillus subtilis subsp. subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T154201 NZ_CP067399:820467-820874 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT154201 WP_003232646.1 NZ_CP051466:c1328349-1328179 [Bacillus subtilis subsp. subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT154201 NZ_CP067399:820220-820486 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8