Detailed information of TA system TA154200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518288..518924
• Replicon: chromosome
• Accession: NZ_CP051466
• Organism: Bacillus subtilis subsp. subtilis strain UCMB5021

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HC658_RS02640
• Protein ID: WP_003156187.1
• Coordinates: 518574..518924 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: HC658_RS02635
• Protein ID: WP_003225183.1
• Coordinates: 518288..518569 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC658_RS02615	514647..515246	-	600	WP_032722928.1	rhomboid family intramembrane serine protease	-
HC658_RS02620	515341..515706	+	366	WP_015252768.1	holo-ACP synthase	-
HC658_RS02625	515872..516888	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
HC658_RS02630	517003..518172	+	1170	WP_032722929.1	alanine racemase	-
HC658_RS02635	518288..518569	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HC658_RS02640	518574..518924	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HC658_RS02645	519039..519863	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
HC658_RS02650	519868..520233	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
HC658_RS02655	520237..520638	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
HC658_RS02660	520650..521657	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
HC658_RS02665	521719..522048	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
HC658_RS02670	522045..522527	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
HC658_RS02675	522493..523281	+	789	WP_029727187.1	RNA polymerase sigma factor SigB	-
HC658_RS02680	523281..523880	+	600	WP_003234303.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T154200 WP_003156187.1 NZ_CP051466:518574-518924 [Bacillus subtilis subsp. subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T154200 NZ_CP067399:714412-714708 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT154200 WP_003225183.1 NZ_CP051466:518288-518569 [Bacillus subtilis subsp. subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT154200 NZ_CP067399:714710-715105 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32