Detailed information of TA system TA154189

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518490..519126
• Replicon: chromosome
• Accession: NZ_CP051464
• Organism: Bacillus mojavensis strain UCMB5075

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HC660_RS02625
• Protein ID: WP_003156187.1
• Coordinates: 518776..519126 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: HC660_RS02620
• Protein ID: WP_003225183.1
• Coordinates: 518490..518771 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC660_RS02600	514845..515444	-	600	WP_168746987.1	rhomboid family intramembrane serine protease	-
HC660_RS02605	515539..515904	+	366	WP_010333111.1	holo-ACP synthase	-
HC660_RS02610	516070..517086	+	1017	WP_081720864.1	outer membrane lipoprotein carrier protein LolA	-
HC660_RS02615	517203..518372	+	1170	WP_168746988.1	alanine racemase	-
HC660_RS02620	518490..518771	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HC660_RS02625	518776..519126	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HC660_RS02630	519243..520067	+	825	WP_081720860.1	RsbT co-antagonist protein RsbRA	-
HC660_RS02635	520072..520437	+	366	WP_010333115.1	RsbT antagonist protein RsbS	-
HC660_RS02640	520441..520842	+	402	WP_010333116.1	serine/threonine-protein kinase RsbT	-
HC660_RS02645	520854..521861	+	1008	WP_010333117.1	phosphoserine phosphatase RsbU	-
HC660_RS02650	521923..522252	+	330	WP_010333118.1	anti-sigma factor antagonist RsbV	-
HC660_RS02655	522249..522731	+	483	WP_010333119.1	anti-sigma B factor RsbW	-
HC660_RS02660	522697..523485	+	789	WP_010333120.1	RNA polymerase sigma factor SigB	-
HC660_RS02665	523485..524084	+	600	WP_010333121.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T154189 WP_003156187.1 NZ_CP051464:518776-519126 [Bacillus mojavensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T154189 NZ_CP067397:c3777004-3776519 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT154189 WP_003225183.1 NZ_CP051464:518490-518771 [Bacillus mojavensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT154189 NZ_CP067397:c3777279-3776992 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32