Detailed information of TA system TA154188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1263710..1264627
• Replicon: chromosome
• Accession: NZ_CP051463
• Organism: Bacillus velezensis strain UCMB5140

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: HC661_RS06695
• Protein ID: WP_007407256.1
• Coordinates: 1263881..1264627 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HC661_RS06690
• Protein ID: WP_003154807.1
• Coordinates: 1263710..1263880 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC661_RS06655	1258934..1260565	+	1632	WP_015417294.1	pyocin knob domain-containing protein	-
HC661_RS06660	1260578..1260949	+	372	WP_015417295.1	YomQ/XkdW protein, phage-like element PBSX	-
HC661_RS06665	1260954..1261151	+	198	WP_012117366.1	XkdX family protein	-
HC661_RS06670	1261208..1261969	+	762	WP_033575307.1	hypothetical protein	-
HC661_RS06675	1262021..1262284	+	264	WP_003154815.1	hemolysin XhlA family protein	-
HC661_RS06680	1262298..1262561	+	264	WP_003154813.1	phage holin	-
HC661_RS06685	1262575..1263453	+	879	WP_015417297.1	N-acetylmuramoyl-L-alanine amidase	-
HC661_RS06690	1263710..1263880	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HC661_RS06695	1263881..1264627	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HC661_RS06700	1264732..1265730	-	999	WP_003154805.1	inorganic phosphate transporter	-
HC661_RS06705	1265743..1266360	-	618	WP_003154804.1	DUF47 domain-containing protein	-
HC661_RS06710	1266646..1267962	-	1317	WP_007610842.1	amino acid permease	-
HC661_RS06715	1268285..1269235	+	951	WP_015417298.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1227723..1301996	74273

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T154188 WP_007407256.1 NZ_CP051463:c1264627-1263881 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T154188 NZ_CP067397:c3667087-3666719 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT154188 WP_003154807.1 NZ_CP051463:c1263880-1263710 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT154188 NZ_CP067397:c3667305-3667084 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14