Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 514142..514779 Replicon chromosome
Accession NZ_CP051463
Organism Bacillus velezensis strain UCMB5140

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HC661_RS02620 Protein ID WP_003156187.1
Coordinates 514429..514779 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag HC661_RS02615 Protein ID WP_003156188.1
Coordinates 514142..514423 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HC661_RS02595 510507..511106 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
HC661_RS02600 511199..511564 + 366 WP_015416872.1 holo-ACP synthase -
HC661_RS02605 511729..512736 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
HC661_RS02610 512853..514022 + 1170 WP_015416873.1 alanine racemase -
HC661_RS02615 514142..514423 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HC661_RS02620 514429..514779 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HC661_RS02625 514897..515718 + 822 WP_015416874.1 STAS domain-containing protein -
HC661_RS02630 515723..516088 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
HC661_RS02635 516091..516492 + 402 WP_003156178.1 anti-sigma regulatory factor -
HC661_RS02640 516504..517511 + 1008 WP_015416875.1 PP2C family protein-serine/threonine phosphatase -
HC661_RS02645 517575..517904 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
HC661_RS02650 517901..518383 + 483 WP_003156173.1 anti-sigma B factor RsbW -
HC661_RS02655 518349..519137 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
HC661_RS02660 519137..519739 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T154187 WP_003156187.1 NZ_CP051463:514429-514779 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T154187 NZ_CP067397:c3618750-3618475 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT154187 WP_003156188.1 NZ_CP051463:514142-514423 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT154187 NZ_CP067397:c3619013-3618753 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References