Detailed information of TA system TA154182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 377833..378419
• Replicon: chromosome
• Accession: NZ_CP051461
• Organism: Polaromonas vacuolata strain KCTC 22033

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HC248_RS01895
• Protein ID: WP_168921029.1
• Coordinates: 378075..378419 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HC248_RS01890
• Protein ID: WP_168921028.1
• Coordinates: 377833..378075 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HC248_RS01865	373143..374339	-	1197	WP_168921023.1	ABC transporter substrate-binding protein	-
HC248_RS01870	374402..375106	-	705	WP_168921024.1	ABC transporter ATP-binding protein	-
HC248_RS01875	375103..375897	-	795	WP_168921025.1	ABC transporter ATP-binding protein	-
HC248_RS01880	376238..377320	+	1083	WP_168921026.1	hypothetical protein	-
HC248_RS01885	377437..377601	+	165	WP_168921027.1	hypothetical protein	-
HC248_RS01890	377833..378075	+	243	WP_168921028.1	PbsX family transcriptional regulator	Antitoxin
HC248_RS01895	378075..378419	+	345	WP_168921029.1	endoribonuclease MazF	Toxin
HC248_RS01900	378485..381808	-	3324	WP_168921030.1	methylmalonyl-CoA mutase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12246.41 Da
• Isoelectric Point: 9.6564

>T154182 WP_168921029.1 NZ_CP051461:378075-378419 [Polaromonas vacuolata]
MATRSYVPDAGDIVLLEFDPQAGHEQAGHRPALVVSPASYNAKTGLMVCCPMSTRIKGHPFEVLVELDAVTSAVLSDQVK
SLDWKVRQAKKKATVPAAVMLHVRAKIKALLAIA

Nucleotide

• Length: 345 bp

>T154182 NZ_CP067397:c1627502-1627218 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8691.02 Da
• Isoelectric Point: 5.7997

>AT154182 WP_168921028.1 NZ_CP051461:377833-378075 [Polaromonas vacuolata]
MESVIRKWGNSLALRLPASALKQADLSLDQKVNIVVSRSRIIIEASGKVEYDLGQLLDAMTAENAHAEADFGKPVGKELL

Nucleotide

• Length: 243 bp

>AT154182 NZ_CP067397:c1627740-1627492 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA