Detailed information of TA system TA154161

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 592549..593333
• Replicon: chromosome
• Accession: NZ_CP051460
• Organism: Dickeya solani strain IFB0421

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HAT92_RS02765
• Protein ID: WP_022632047.1
• Coordinates: 592839..593333 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2K8W1V4
• Locus tag: HAT92_RS02760
• Protein ID: WP_022632046.1
• Coordinates: 592549..592842 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAT92_RS02740	588375..588827	+	453	WP_022632042.1	hypothetical protein	-
HAT92_RS02745	588870..589166	+	297	WP_022632043.1	hypothetical protein	-
HAT92_RS02750	589194..589901	+	708	WP_022632044.1	SDR family oxidoreductase	-
HAT92_RS02755	590893..592144	-	1252	Protein_534	tyrosine-type recombinase/integrase	-
HAT92_RS02760	592549..592842	+	294	WP_022632046.1	DUF1778 domain-containing protein	Antitoxin
HAT92_RS02765	592839..593333	+	495	WP_022632047.1	GNAT family N-acetyltransferase	Toxin
HAT92_RS02770	593385..593782	+	398	Protein_537	DUF3577 domain-containing protein	-
HAT92_RS02775	593898..595169	+	1272	WP_022632048.1	hypothetical protein	-
HAT92_RS02780	595173..595745	+	573	WP_022632049.1	hypothetical protein	-
HAT92_RS02785	595733..597673	+	1941	WP_022632050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	592306..602631	10325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17639.41 Da
• Isoelectric Point: 6.8524

>T154161 WP_022632047.1 NZ_CP051460:592839-593333 [Dickeya solani]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSRVLAYYSLASSAVATTAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARDFYLRVGFEPSPIDPMILMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T154161 NZ_CP067373:11345-11761 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10942.53 Da
• Isoelectric Point: 9.8078

>AT154161 WP_022632046.1 NZ_CP051460:592549-592842 [Dickeya solani]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT154161 NZ_CP067373:11118-11348 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W1V4