Detailed information of TA system TA154148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1882262..1882815
• Replicon: chromosome
• Accession: NZ_CP051458
• Organism: Dickeya solani strain IFB0231

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HAT89_RS08355
• Protein ID: WP_022633101.1
• Coordinates: 1882501..1882815 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2K8W593
• Locus tag: HAT89_RS08350
• Protein ID: WP_022633100.1
• Coordinates: 1882262..1882498 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAT89_RS08330	1877838..1879487	+	1650	WP_022633097.1	hypothetical protein	-
HAT89_RS08335	1879848..1880249	-	402	WP_023637748.1	hypothetical protein	-
HAT89_RS08340	1880313..1880606	-	294	WP_146053334.1	hypothetical protein	-
HAT89_RS08345	1880997..1881941	+	945	WP_022633099.1	DUF1738 domain-containing protein	-
HAT89_RS08350	1882262..1882498	+	237	WP_022633100.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HAT89_RS08355	1882501..1882815	+	315	WP_022633101.1	CcdB family protein	Toxin
HAT89_RS08360	1882812..1883069	-	258	WP_022633102.1	hypothetical protein	-
HAT89_RS08365	1883245..1884552	-	1308	WP_022633103.1	type II toxin-antitoxin system HipA family toxin	-
HAT89_RS08370	1884552..1884959	-	408	WP_071598628.1	helix-turn-helix transcriptional regulator	-
HAT89_RS08375	1885342..1886088	-	747	WP_022633104.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	hcp	1873279..1921657	48378

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11459.38 Da
• Isoelectric Point: 7.9859

>T154148 WP_022633101.1 NZ_CP051458:1882501-1882815 [Dickeya solani]
MQFTVYGNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPVEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGI

Nucleotide

• Length: 315 bp

>T154148 NZ_CP067371:53928-54410 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9020.06 Da
• Isoelectric Point: 6.2206

>AT154148 WP_022633100.1 NZ_CP051458:1882262-1882498 [Dickeya solani]
MTAKQRNTQSVTMTVERALLVRAREAGINLSAILTTALDAELRHHEAKKWQEENREAIDALNRFHDENGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT154148 NZ_CP067371:53674-53940 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W593