Detailed information of TA system TA154142

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 592561..593345
• Replicon: chromosome
• Accession: NZ_CP051458
• Organism: Dickeya solani strain IFB0231

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HAT89_RS02760
• Protein ID: WP_022632047.1
• Coordinates: 592851..593345 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2K8W1V4
• Locus tag: HAT89_RS02755
• Protein ID: WP_022632046.1
• Coordinates: 592561..592854 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAT89_RS02735	588387..588839	+	453	WP_022632042.1	hypothetical protein	-
HAT89_RS02740	588882..589178	+	297	WP_022632043.1	hypothetical protein	-
HAT89_RS02745	589206..589913	+	708	WP_022632044.1	SDR family oxidoreductase	-
HAT89_RS02750	590905..592156	-	1252	Protein_533	tyrosine-type recombinase/integrase	-
HAT89_RS02755	592561..592854	+	294	WP_022632046.1	DUF1778 domain-containing protein	Antitoxin
HAT89_RS02760	592851..593345	+	495	WP_022632047.1	GNAT family N-acetyltransferase	Toxin
HAT89_RS02765	593397..593794	+	398	Protein_536	DUF3577 domain-containing protein	-
HAT89_RS02770	593910..595181	+	1272	WP_022632048.1	hypothetical protein	-
HAT89_RS02775	595185..595757	+	573	WP_022632049.1	hypothetical protein	-
HAT89_RS02780	595745..597685	+	1941	WP_022632050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	592318..602643	10325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17639.41 Da
• Isoelectric Point: 6.8524

>T154142 WP_022632047.1 NZ_CP051458:592851-593345 [Dickeya solani]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSRVLAYYSLASSAVATTAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARDFYLRVGFEPSPIDPMILMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T154142 NZ_CP067370:4242473-4242850 [Klebsiella pneumoniae]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCGTTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10942.53 Da
• Isoelectric Point: 9.8078

>AT154142 WP_022632046.1 NZ_CP051458:592561-592854 [Dickeya solani]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT154142 NZ_CP067370:4242063-4242422 [Klebsiella pneumoniae]
TTGTCGAATAAAACACCGATAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGGCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
CGGTTTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W1V4