Detailed information of TA system TA154123

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 592560..593344
• Replicon: chromosome
• Accession: NZ_CP051457
• Organism: Dickeya solani strain IFB0167

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HAT87_RS02765
• Protein ID: WP_022632047.1
• Coordinates: 592850..593344 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2K8W1V4
• Locus tag: HAT87_RS02760
• Protein ID: WP_022632046.1
• Coordinates: 592560..592853 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAT87_RS02740	588386..588838	+	453	WP_022632042.1	hypothetical protein	-
HAT87_RS02745	588881..589177	+	297	WP_022632043.1	hypothetical protein	-
HAT87_RS02750	589205..589912	+	708	WP_022632044.1	SDR family oxidoreductase	-
HAT87_RS02755	590904..592155	-	1252	Protein_534	tyrosine-type recombinase/integrase	-
HAT87_RS02760	592560..592853	+	294	WP_022632046.1	DUF1778 domain-containing protein	Antitoxin
HAT87_RS02765	592850..593344	+	495	WP_022632047.1	GNAT family N-acetyltransferase	Toxin
HAT87_RS02770	593396..593793	+	398	Protein_537	DUF3577 domain-containing protein	-
HAT87_RS02775	593909..595180	+	1272	WP_022632048.1	hypothetical protein	-
HAT87_RS02780	595184..595756	+	573	WP_022632049.1	hypothetical protein	-
HAT87_RS02785	595744..597684	+	1941	WP_022632050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	592317..602642	10325

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17639.41 Da
• Isoelectric Point: 6.8524

>T154123 WP_022632047.1 NZ_CP051457:592850-593344 [Dickeya solani]
MISAPEPLHAEHVLSSFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDDSRVLAYYSLASSAVATTAAPGRFRRNMPDPI
PVVVLGRLAVDTSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEARDFYLRVGFEPSPIDPMILMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T154123 NZ_CP067343:10094-10342 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10942.53 Da
• Isoelectric Point: 9.8078

>AT154123 WP_022632046.1 NZ_CP051457:592560-592853 [Dickeya solani]
MPVTNSVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPEPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT154123 NZ_CP067343:10339-10686 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2K8W1V4