Detailed information of TA system TA154119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 34333..34976
• Replicon: plasmid pSCLE3
• Accession: NZ_CP051433
• Organism: Escherichia sp. SCLE84

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A6H2GRP8
• Locus tag: HGD79_RS25925
• Protein ID: WP_023292131.1
• Coordinates: 34333..34749 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6B7Q5S4
• Locus tag: HGD79_RS25930
• Protein ID: WP_021312476.1
• Coordinates: 34746..34976 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGD79_RS25880	29683..29991	+	309	Protein_26	DDE-type integrase/transposase/recombinase	-
HGD79_RS25885	30021..30230	-	210	WP_094308932.1	hypothetical protein	-
HGD79_RS25890	30279..30482	-	204	WP_004150739.1	hemolysin expression modulator Hha	-
HGD79_RS25895	30543..31037	-	495	WP_094313750.1	DNA-binding protein	-
HGD79_RS25900	31068..31640	-	573	WP_094309628.1	hypothetical protein	-
HGD79_RS25905	31645..31893	-	249	WP_065801593.1	hypothetical protein	-
HGD79_RS25910	32172..33178	-	1007	Protein_32	IS110 family transposase	-
HGD79_RS25915	33735..33989	-	255	WP_095153353.1	hypothetical protein	-
HGD79_RS25920	34053..34298	-	246	WP_065801591.1	hypothetical protein	-
HGD79_RS25925	34333..34749	-	417	WP_023292131.1	type II toxin-antitoxin system VapC family toxin	Toxin
HGD79_RS25930	34746..34976	-	231	WP_021312476.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HGD79_RS25935	35552..35902	+	351	WP_000493379.1	hypothetical protein	-
HGD79_RS25940	35946..36155	+	210	Protein_38	hypothetical protein	-
HGD79_RS25945	36631..37422	+	792	WP_112907394.1	site-specific integrase	-
HGD79_RS25950	37560..37835	-	276	WP_000239529.1	hypothetical protein	-
HGD79_RS25955	37829..38473	-	645	WP_000633913.1	AAA family ATPase	-
HGD79_RS25960	38702..39673	+	972	WP_001103690.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	vat / cseA	1..96595	96595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14975.40 Da
• Isoelectric Point: 8.5464

>T154119 WP_023292131.1 NZ_CP051433:c34749-34333 [Escherichia sp. SCLE84]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T154119 NZ_CP067342:3701491-3701769 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.76 Da
• Isoelectric Point: 4.3410

>AT154119 WP_021312476.1 NZ_CP051433:c34976-34746 [Escherichia sp. SCLE84]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT154119 NZ_CP067342:3701228-3701488 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A6H2GRP8

Antitoxin

• AlphaFold DB: A0A6B7Q5S4