Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 129909..130552 Replicon plasmid pSCLE1
Accession NZ_CP051431
Organism Escherichia sp. SCLE84

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HGD79_RS25000 Protein ID WP_001044768.1
Coordinates 129909..130325 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HGD79_RS25005 Protein ID WP_001261287.1
Coordinates 130322..130552 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGD79_RS24985 126408..126998 - 591 WP_000194575.1 hypothetical protein -
HGD79_RS24990 126998..127255 - 258 WP_000343085.1 hypothetical protein -
HGD79_RS24995 127609..129747 + 2139 WP_000350638.1 AAA family ATPase -
HGD79_RS25000 129909..130325 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HGD79_RS25005 130322..130552 - 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HGD79_RS25010 130848..131120 + 273 WP_061602529.1 hypothetical protein -
HGD79_RS25015 131167..131871 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
HGD79_RS25020 131918..132352 - 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
HGD79_RS25025 132424..132774 + 351 WP_001294663.1 mercuric transport protein MerT -
HGD79_RS25030 132788..133063 + 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
HGD79_RS25035 133099..133521 + 423 WP_001340589.1 organomercurial transporter MerC -
HGD79_RS25040 133573..135267 + 1695 WP_000105636.1 mercury(II) reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-14 / fosA3 / aac(3)-IVa / mef(B) / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / qnrS1 / tet(A) - 1..217533 217533
- flank IS/Tn - - 131167..131871 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T154116 WP_001044768.1 NZ_CP051431:c130325-129909 [Escherichia sp. SCLE84]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T154116 NZ_CP067342:3497390-3497608 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT154116 WP_001261287.1 NZ_CP051431:c130552-130322 [Escherichia sp. SCLE84]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT154116 NZ_CP067342:3496990-3497364 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References