Detailed information of TA system TA154115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 85773..87396
• Replicon: plasmid pSCLE1
• Accession: NZ_CP051431
• Organism: Escherichia sp. SCLE84

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HGD79_RS24745
• Protein ID: WP_086722013.1
• Coordinates: 86071..87396 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: HGD79_RS24740
• Protein ID: WP_024136327.1
• Coordinates: 85773..86066 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGD79_RS24715	81645..82886	+	1242	WP_001053338.1	VWA domain-containing protein	-
HGD79_RS24720	83449..83730	-	282	WP_000077926.1	hypothetical protein	-
HGD79_RS24725	83780..83971	-	192	WP_000398480.1	hypothetical protein	-
HGD79_RS24730	84063..84404	-	342	WP_001151575.1	hypothetical protein	-
HGD79_RS24735	84777..85169	+	393	WP_000341066.1	hypothetical protein	-
HGD79_RS24740	85773..86066	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
HGD79_RS24745	86071..87396	+	1326	WP_086722013.1	type II toxin-antitoxin system HipA family toxin	Toxin
HGD79_RS24750	87457..87663	+	207	WP_000134171.1	hypothetical protein	-
HGD79_RS24755	87765..88175	+	411	WP_000985911.1	hypothetical protein	-
HGD79_RS24760	88188..89003	+	816	WP_001572439.1	HNH endonuclease	-
HGD79_RS24765	89257..89682	+	426	WP_001043843.1	hypothetical protein	-
HGD79_RS24770	90136..90372	+	237	Protein_88	hypothetical protein	-
HGD79_RS24775	90427..90726	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-14 / fosA3 / aac(3)-IVa / mef(B) / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / qnrS1 / tet(A)	-	1..217533	217533

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50263.22 Da
• Isoelectric Point: 6.1932

>T154115 WP_086722013.1 NZ_CP051431:86071-87396 [Escherichia sp. SCLE84]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEERWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T154115 NZ_CP067342:2745181-2745288 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT154115 WP_024136327.1 NZ_CP051431:85773-86066 [Escherichia sp. SCLE84]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT154115 NZ_CP067342:c2745134-2745068 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1