Detailed information of TA system TA154113

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 4284803..4285386
• Replicon: chromosome
• Accession: NZ_CP051430
• Organism: Escherichia sp. SCLE84

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S1EZP4
• Locus tag: HGD79_RS20750
• Protein ID: WP_000254738.1
• Coordinates: 4285051..4285386 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: HGD79_RS20745
• Protein ID: WP_000581937.1
• Coordinates: 4284803..4285051 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGD79_RS20735	4281142..4282443	+	1302	WP_000046790.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
HGD79_RS20740	4282491..4284725	+	2235	WP_000226815.1	GTP pyrophosphokinase	-
HGD79_RS20745	4284803..4285051	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HGD79_RS20750	4285051..4285386	+	336	WP_000254738.1	endoribonuclease MazF	Toxin
HGD79_RS20755	4285457..4286248	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
HGD79_RS20760	4286476..4288113	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
HGD79_RS20765	4288201..4289499	+	1299	WP_168848490.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T154113 WP_000254738.1 NZ_CP051430:4285051-4285386 [Escherichia sp. SCLE84]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T154113 NZ_CP067342:c2536622-2536446 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT154113 WP_000581937.1 NZ_CP051430:4284803-4285051 [Escherichia sp. SCLE84]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT154113 NZ_CP067342:c2536400-2535984 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CK9
• PDB: 5CKH
• PDB: 5CO7
• PDB: 3NFC
• PDB: 5CR2
• PDB: 5CKB
• PDB: 5CQY
• PDB: 5CKD
• PDB: 5CKE
• PDB: 5CKF

Antitoxin

• PDB: 1UB4
• PDB: 5CQX
• PDB: 5CQY
• PDB: 1MVF
• PDB: 2MRN
• PDB: 2MRU
• AlphaFold DB: A0A7U9LMB4