Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2739903..2740400 Replicon chromosome
Accession NZ_CP051430
Organism Escherichia sp. SCLE84

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HGD79_RS13395 Protein ID WP_001173343.1
Coordinates 2739903..2740076 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HGD79_RS13400 Protein ID WP_000398619.1
Coordinates 2740104..2740400 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGD79_RS13385 2736573..2738030 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
HGD79_RS13390 2738267..2739784 + 1518 WP_001295074.1 lysine--tRNA ligase -
HGD79_RS13395 2739903..2740076 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HGD79_RS13400 2740104..2740400 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HGD79_RS13405 2740627..2740899 - 273 WP_000405647.1 N-acetyltransferase -
HGD79_RS13410 2740911..2741141 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
HGD79_RS13415 2741322..2742953 + 1632 WP_001216487.1 sensor histidine kinase -
HGD79_RS13420 2742950..2743669 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T154108 WP_001173343.1 NZ_CP051430:c2740076-2739903 [Escherichia sp. SCLE84]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T154108 NZ_CP067342:1921580-1921831 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCGCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT154108 WP_000398619.1 NZ_CP051430:c2740400-2740104 [Escherichia sp. SCLE84]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT154108 NZ_CP067342:1921828-1922082 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References