Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1963695..1964532 Replicon chromosome
Accession NZ_CP051430
Organism Escherichia sp. SCLE84

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HGD79_RS09740 Protein ID WP_000227784.1
Coordinates 1963990..1964532 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HGD79_RS09735 Protein ID WP_001297137.1
Coordinates 1963695..1964006 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGD79_RS09710 1958715..1959662 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HGD79_RS09715 1959684..1961675 + 1992 WP_168848377.1 cytochrome o ubiquinol oxidase subunit I -
HGD79_RS09720 1961665..1962279 + 615 WP_000179821.1 cytochrome o ubiquinol oxidase subunit III -
HGD79_RS09725 1962279..1962608 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HGD79_RS09730 1962620..1963510 + 891 WP_000971336.1 heme o synthase -
HGD79_RS09735 1963695..1964006 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HGD79_RS09740 1963990..1964532 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HGD79_RS09745 1964588..1965523 - 936 WP_001297127.1 sel1 repeat family protein -
HGD79_RS09750 1965931..1967295 + 1365 WP_001000960.1 MFS transporter -
HGD79_RS09755 1967423..1967914 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HGD79_RS09760 1968082..1968993 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T154105 WP_000227784.1 NZ_CP051430:1963990-1964532 [Escherichia sp. SCLE84]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T154105 NZ_CP067342:c852165-851788 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCGCGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCGGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT154105 WP_001297137.1 NZ_CP051430:1963695-1964006 [Escherichia sp. SCLE84]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT154105 NZ_CP067342:c852623-852255 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCAGACCGTGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References