Detailed information of TA system TA154080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1553132..1553885
• Replicon: chromosome
• Accession: NZ_CP051429
• Organism: Dickeya dianthicola strain 67-19

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: HGI48_RS07290
• Protein ID: WP_192990817.1
• Coordinates: 1553132..1553389 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: HGI48_RS07295
• Protein ID: WP_192990818.1
• Coordinates: 1553397..1553885 (+)
• Length: 163 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGI48_RS07275	1548337..1549545	-	1209	WP_192990815.1	putative DNA binding domain-containing protein	-
HGI48_RS07280	1549909..1552059	-	2151	WP_192990816.1	formate dehydrogenase subunit alpha	-
HGI48_RS07285	1552094..1552636	-	543	WP_022632926.1	4Fe-4S dicluster domain-containing protein	-
HGI48_RS07290	1553132..1553389	+	258	WP_192990817.1	DUF4160 domain-containing protein	Toxin
HGI48_RS07295	1553397..1553885	+	489	WP_192990818.1	addiction module antitoxin	Antitoxin
HGI48_RS07300	1553972..1554469	-	498	WP_192990819.1	hydrogenase maturation peptidase HycI	-
HGI48_RS07305	1554462..1554860	-	399	WP_192990820.1	formate hydrogenlyase maturation HycH family protein	-
HGI48_RS07310	1554857..1555648	-	792	WP_192990821.1	NADH-quinone oxidoreductase subunit B family protein	-
HGI48_RS07315	1555645..1556214	-	570	WP_192990822.1	hydrogenase 4 subunit H	-
HGI48_RS07320	1556225..1557952	-	1728	WP_192990823.1	hydrogenase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

• Uncharacterized protein HI1420 (80-157)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10020.63 Da
• Isoelectric Point: 9.4806

>T154080 WP_192990817.1 NZ_CP051429:1553132-1553389 [Dickeya dianthicola]
MPEIDRLAGLIFHLYFYDDHRHHLPHIHVCYGNHELIINIRTGTEIEGYLPNNKRKLALAHIRAYRQPLLSMWERAVVGR
NPGKL

Nucleotide

• Length: 258 bp

>T154080 NZ_CP051429:1553132-1553389 [Dickeya dianthicola]
ATGCCTGAGATCGACAGACTGGCGGGGCTGATTTTCCATTTGTACTTTTATGACGATCATCGGCACCACTTGCCGCATAT
CCATGTCTGCTACGGCAACCATGAACTGATCATCAATATTCGTACCGGAACCGAGATTGAAGGATATCTGCCGAATAACA
AGCGCAAGCTGGCGCTTGCACATATCAGGGCCTATCGGCAACCGTTGCTGAGTATGTGGGAACGGGCGGTAGTGGGGAGA
AATCCGGGAAAACTGTGA

Antitoxin

Protein

• Length: 163 a.a.
• Molecular weight: 18028.63 Da
• Isoelectric Point: 7.0619

>AT154080 WP_192990818.1 NZ_CP051429:1553397-1553885 [Dickeya dianthicola]
MHKIVEVDVIGDFSLRLTYEDGLVCDINLYHYAPSPVFSNASQFIKFGLLPDGSLEWLPDIKISASELRKKGEYVSHTLA
VRERNFADVISRAFYDAVIENRPEILQAALKIAVEKHGNSTVAKEAGAKSRTSLYKSLNKNTHVSLATVVSLAHTVLQLE
EK

Nucleotide

• Length: 489 bp

>AT154080 NZ_CP051429:1553397-1553885 [Dickeya dianthicola]
ATGCATAAAATTGTAGAAGTTGACGTCATTGGCGATTTTTCGTTGCGCCTGACCTATGAAGATGGTCTGGTTTGCGACAT
CAATCTTTATCACTATGCCCCCAGCCCGGTATTTTCGAACGCCAGCCAATTTATCAAGTTTGGGTTGCTGCCGGACGGCT
CGCTGGAATGGCTGCCGGATATTAAGATTTCCGCGTCTGAATTGCGGAAAAAAGGGGAATATGTCAGCCACACGCTGGCG
GTGCGAGAACGGAACTTCGCTGATGTCATCTCTCGCGCTTTCTATGATGCCGTGATTGAAAACCGGCCGGAAATTCTGCA
AGCGGCGTTGAAAATTGCGGTGGAAAAACATGGCAACAGCACGGTGGCGAAAGAGGCGGGGGCGAAAAGCCGCACCAGTC
TGTATAAGTCGCTGAATAAGAATACCCATGTCAGCCTGGCGACGGTGGTCAGCCTGGCGCATACCGTGCTGCAGTTGGAG
GAGAAGTAG