Detailed information of TA system TA154076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 989158..989942
• Replicon: chromosome
• Accession: NZ_CP051429
• Organism: Dickeya dianthicola strain 67-19

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HGI48_RS04715
• Protein ID: WP_192991409.1
• Coordinates: 989448..989942 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: HGI48_RS04710
• Protein ID: WP_192990491.1
• Coordinates: 989158..989451 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGI48_RS04680	984842..985567	+	726	WP_192990487.1	Expansin-YoaJ	-
HGI48_RS04685	985638..986585	+	948	WP_192990488.1	glycoside hydrolase family 5 protein	-
HGI48_RS04690	986697..986858	-	162	WP_161132386.1	CcdB family protein	-
HGI48_RS04695	986867..987004	-	138	Protein_914	molybdopterin-guanine dinucleotide biosynthesis protein MobC	-
HGI48_RS04700	987515..988168	-	654	WP_192990489.1	RES family NAD+ phosphorylase	-
HGI48_RS04705	988161..988880	-	720	WP_192990490.1	hypothetical protein	-
HGI48_RS04710	989158..989451	+	294	WP_192990491.1	DUF1778 domain-containing protein	Antitoxin
HGI48_RS04715	989448..989942	+	495	WP_192991409.1	GNAT family N-acetyltransferase	Toxin
HGI48_RS04720	990158..990442	+	285	WP_168640622.1	hypothetical protein	-
HGI48_RS04725	990445..990563	+	119	Protein_920	HTH domain-containing protein	-
HGI48_RS04730	991378..992454	+	1077	WP_192990492.1	hypothetical protein	-
HGI48_RS04735	992485..993561	+	1077	WP_192990493.1	hypothetical protein	-
HGI48_RS04740	993581..994276	+	696	WP_192990494.1	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	984842..1020927	36085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17756.60 Da
• Isoelectric Point: 7.2151

>T154076 WP_192991409.1 NZ_CP051429:989448-989942 [Dickeya dianthicola]
MISAPEPLRAEHVLSSFCCGVESMDNWLKQRAMKNQLTGASRTFVSCDDSKVLAYYSLASSAVATNAAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQGVGRALVRDAGLRVIQVADTIGIRGMLVHALSDEAREFYLRVGFEPSPIDPMILMVTLGDLVD
SLSI

Nucleotide

• Length: 495 bp

>T154076 NZ_CP067311:307379-307482 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10887.46 Da
• Isoelectric Point: 10.4623

>AT154076 WP_192990491.1 NZ_CP051429:989158-989451 [Dickeya dianthicola]
MSATNRVSAKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT154076 NZ_CP067311:c307520-307249 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT