Detailed information of TA system TA154074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 449328..450070
• Replicon: chromosome
• Accession: NZ_CP051429
• Organism: Dickeya dianthicola strain 67-19

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HGI48_RS02040
• Protein ID: WP_192990114.1
• Coordinates: 449328..449807 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: E0SJ01
• Locus tag: HGI48_RS02045
• Protein ID: WP_013316118.1
• Coordinates: 449798..450070 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGI48_RS02020	445182..445542	+	361	Protein_389	type II toxin-antitoxin system RelE/ParE family toxin	-
HGI48_RS02025	445526..445849	+	324	WP_095104210.1	DNA-binding transcriptional regulator	-
HGI48_RS02030	446016..447320	+	1305	WP_095104209.1	SIR2 family protein	-
HGI48_RS02035	447320..449041	+	1722	WP_192990113.1	ATP-binding protein	-
HGI48_RS02040	449328..449807	-	480	WP_192990114.1	GNAT family N-acetyltransferase	Toxin
HGI48_RS02045	449798..450070	-	273	WP_013316118.1	DUF1778 domain-containing protein	Antitoxin
HGI48_RS02050	450519..451219	+	701	Protein_395	integrase family protein	-
HGI48_RS02055	451467..451772	+	306	WP_192990115.1	helix-turn-helix domain-containing protein	-
HGI48_RS02060	451784..452839	+	1056	Protein_397	type II toxin-antitoxin system HipA family toxin	-
HGI48_RS02065	452862..453134	-	273	WP_192990116.1	DUF1778 domain-containing protein	-
HGI48_RS02070	453541..454929	+	1389	WP_192990117.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	438156..451219	13063

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17554.34 Da
• Isoelectric Point: 9.5367

>T154074 WP_192990114.1 NZ_CP051429:c449807-449328 [Dickeya dianthicola]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPILVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVASEIQEHTLLLSLW

Nucleotide

• Length: 480 bp

>T154074 NZ_CP067309:2042-2320 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAACGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGTGTATTGCATACCGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10384.88 Da
• Isoelectric Point: 4.6702

>AT154074 WP_013316118.1 NZ_CP051429:c450070-449798 [Dickeya dianthicola]
MRTEPKEMPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRVN
ALLNRKSPWE

Nucleotide

• Length: 273 bp

>AT154074 NZ_CP067309:1773-2042 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCCTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGCCGCAGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: E0SJ01