Detailed information of TA system TA154062

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4331328..4331986
• Replicon: chromosome
• Accession: NZ_CP051427
• Organism: Klebsiella michiganensis strain X2-1

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HGI13_RS20570
• Protein ID: WP_209744128.1
• Coordinates: 4331328..4331648 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HGI13_RS20575
• Protein ID: WP_209744132.1
• Coordinates: 4331669..4331986 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGI13_RS20555	4329486..4330055	+	570	WP_049091442.1	recombinase family protein	-
HGI13_RS20560	4330302..4330541	-	240	WP_015370245.1	DinI family protein	-
HGI13_RS20565	4330622..4331005	+	384	WP_049091441.1	S24 family peptidase	-
HGI13_RS20570	4331328..4331648	-	321	WP_209744128.1	TA system toxin CbtA family protein	Toxin
HGI13_RS20575	4331669..4331986	-	318	WP_209744132.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HGI13_RS20580	4332005..4332226	-	222	WP_209744136.1	DUF987 domain-containing protein	-
HGI13_RS20585	4332235..4332711	-	477	Protein_4049	RadC family protein	-
HGI13_RS20590	4332724..4333182	-	459	WP_209744139.1	antirestriction protein	-
HGI13_RS20595	4333262..4333498	-	237	Protein_4051	DUF905 domain-containing protein	-
HGI13_RS20600	4333576..4333986	-	411	WP_139949634.1	hypothetical protein	-
HGI13_RS20605	4334075..4334689	-	615	WP_209744142.1	hypothetical protein	-
HGI13_RS20610	4334686..4335390	-	705	WP_209744144.1	WYL domain-containing protein	-
HGI13_RS20615	4335592..4336425	-	834	WP_209744147.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4262215..4336425	74210

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-104)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12134.14 Da
• Isoelectric Point: 7.8586

>T154062 WP_209744128.1 NZ_CP051427:c4331648-4331328 [Klebsiella michiganensis]
MKTLPATTLRTAKPCPSTVIVWQMLLSRLLDQHYGLTLNDTPFSDETVIKEHIDAGISLADAINFLVEKYELIRIDRKGC
SWQEQSPFITAVDILKARRAMREMRT

Nucleotide

• Length: 321 bp

>T154062 NZ_CP067307:c3796768-3796433 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAAAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11920.65 Da
• Isoelectric Point: 8.1359

>AT154062 WP_209744132.1 NZ_CP051427:c4331986-4331669 [Klebsiella michiganensis]
MSNITWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECLKLDVAFPHFIRQMKSMLTSGEMNPRYAHCVTLYH
NGFTCEADTLGSCGYVYITVYPTQR

Nucleotide

• Length: 318 bp

>AT154062 NZ_CP067307:c3797016-3796768 [Escherichia coli]
ATGATCCACAGTAGCATAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA