154060

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 2312461..2313242 Replicon chromosome
Accession NZ_CP051427
Organism Klebsiella michiganensis strain X2-1

Toxin (Protein)


Gene name PP_4152 Uniprot ID -
Locus tag HGI13_RS27950 Protein ID WP_228294056.1
Coordinates 2312461..2312781 (-) Length 107 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID -
Locus tag HGI13_RS10835 Protein ID WP_042927199.1
Coordinates 2312781..2313242 (-) Length 154 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGI13_RS10815 2307942..2309303 + 1362 WP_209745741.1 integrating conjugative element protein -
HGI13_RS10820 2309300..2310802 + 1503 WP_209745472.1 conjugal transfer protein TraG N-terminal domain-containing protein -
HGI13_RS10825 2310899..2311771 + 873 WP_209745475.1 HNH endonuclease -
HGI13_RS27945 2312206..2312460 - 255 WP_009652311.1 hypothetical protein -
HGI13_RS27950 2312461..2312781 - 321 WP_228294056.1 PIN domain-containing protein Toxin
HGI13_RS10835 2312781..2313242 - 462 WP_042927199.1 helix-turn-helix domain-containing protein Antitoxin
HGI13_RS10840 2313477..2313830 + 354 Protein_2150 HNH endonuclease -
HGI13_RS10845 2313869..2314075 - 207 WP_227500426.1 cell surface composition regulator GlgS -
HGI13_RS10850 2314403..2314693 + 291 WP_025713656.1 hypothetical protein -
HGI13_RS10855 2314758..2315663 + 906 WP_209745481.1 zincin-like metallopeptidase domain-containing protein -
HGI13_RS10860 2315728..2316099 + 372 WP_049152373.1 hypothetical protein -
HGI13_RS10865 2316174..2316632 - 459 WP_179291259.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2259675..2324923 65248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-103)

Antitoxin

SinI-like, DNA-binding domain

(84-130)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 12099.87 Da        Isoelectric Point: 4.6266

>T154060 WP_228294056.1 NZ_CP051427:c2312781-2312461 [Klebsiella michiganensis]
MNHSPYPVVLDACVLYPSLLRDLLMRLGLKGLYQPKWTAEIQDEWQRNLLHDRSDLTPEQVKRTDQLMNEAIPDAMVTGY
ENLIASIELPDLDDRHVVAAAVRCNA

Download         Length: 321 bp

>T154060 NZ_CP067307:c3001855-3001604 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 154 a.a.        Molecular weight: 17021.52 Da        Isoelectric Point: 5.9939

>AT154060 WP_042927199.1 NZ_CP051427:c2313242-2312781 [Klebsiella michiganensis]
MTNSFLDSLSLPAPKEIEAAVRGQRELATYLSTKLETQKISIQDADNVSHQIELPTSSLTLLMAILGELAAGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEDGELPFHKTGRHRRVLFADLMKYKELRDQKSQKAMQELSDLSQDLGLY

Download         Length: 462 bp

>AT154060 NZ_CP067307:c3001607-3001353 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References