Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1563621..1564240 Replicon chromosome
Accession NZ_CP051427
Organism Klebsiella michiganensis strain X2-1

Toxin (Protein)


Gene name Hha Uniprot ID J5X5R4
Locus tag HGI13_RS07350 Protein ID WP_004848333.1
Coordinates 1564022..1564240 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J4WQ74
Locus tag HGI13_RS07345 Protein ID WP_009653563.1
Coordinates 1563621..1563995 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HGI13_RS07335 1558780..1559973 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HGI13_RS07340 1559996..1563142 + 3147 WP_004848339.1 multidrug efflux RND transporter permease subunit AcrB -
HGI13_RS07345 1563621..1563995 + 375 WP_009653563.1 Hha toxicity modulator TomB Antitoxin
HGI13_RS07350 1564022..1564240 + 219 WP_004848333.1 HHA domain-containing protein Toxin
HGI13_RS07355 1564402..1564968 + 567 WP_209745220.1 maltose O-acetyltransferase -
HGI13_RS27915 1564940..1565074 - 135 WP_223226670.1 hypothetical protein -
HGI13_RS07360 1565095..1565565 + 471 WP_004848329.1 YlaC family protein -
HGI13_RS07365 1565540..1566994 - 1455 WP_086530504.1 PLP-dependent aminotransferase family protein -
HGI13_RS07370 1567096..1567794 + 699 WP_182409484.1 GNAT family protein -
HGI13_RS07375 1567791..1567931 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HGI13_RS07380 1567931..1568194 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8626.03 Da        Isoelectric Point: 8.9008

>T154058 WP_004848333.1 NZ_CP051427:1564022-1564240 [Klebsiella michiganensis]
MSDKTLTKVDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T154058 NZ_CP067307:c2785636-2785533 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14378.14 Da        Isoelectric Point: 4.8989

>AT154058 WP_009653563.1 NZ_CP051427:1563621-1563995 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPVSLSC

Download         Length: 375 bp

>AT154058 NZ_CP067307:2785495-2785766 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R4L0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R4I8

References