Detailed information of TA system TA154050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 86545..87070
• Replicon: plasmid p20762-1
• Accession: NZ_CP051405
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain CVM 20762

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: HFQ58_RS23695
• Protein ID: WP_001159871.1
• Coordinates: 86765..87070 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: H9TJP1
• Locus tag: HFQ58_RS23690
• Protein ID: WP_000813630.1
• Coordinates: 86545..86763 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFQ58_RS23660	81546..82136	-	591	WP_000194550.1	hypothetical protein	-
HFQ58_RS23665	82136..82390	-	255	WP_000343103.1	hypothetical protein	-
HFQ58_RS23670	82742..83743	+	1002	WP_000774875.1	DUF4238 domain-containing protein	-
HFQ58_RS23675	83919..84263	-	345	WP_000793307.1	hypothetical protein	-
HFQ58_RS23680	84762..85016	-	255	WP_000678527.1	hypothetical protein	-
HFQ58_RS23685	85061..85987	-	927	WP_001290416.1	hypothetical protein	-
HFQ58_RS23690	86545..86763	+	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HFQ58_RS23695	86765..87070	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
HFQ58_RS23700	87071..87877	+	807	WP_000016969.1	site-specific integrase	-
HFQ58_RS23705	88057..88701	+	645	WP_001144036.1	ParA family protein	-
HFQ58_RS23710	88788..89096	+	309	WP_000030199.1	hypothetical protein	-
HFQ58_RS23715	89510..90490	+	981	WP_000688514.1	plasmid segregation protein ParM	-
HFQ58_RS23720	90483..90899	+	417	WP_001278818.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99003	99003

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T154050 WP_001159871.1 NZ_CP051405:86765-87070 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T154050 NZ_CP067307:c1581587-1581201 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAAAGGATTTGATCGAAACCGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT154050 WP_000813630.1 NZ_CP051405:86545-86763 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT154050 NZ_CP067307:c1581211-1580882 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A829CEF5