Detailed information of TA system TA154035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 66446..66971
• Replicon: plasmid p24362-2
• Accession: NZ_CP051377
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain CVM 24362

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: I3W3D5
• Locus tag: HFS07_RS00425
• Protein ID: WP_001159863.1
• Coordinates: 66666..66971 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: M7S5D0
• Locus tag: HFS07_RS00420
• Protein ID: WP_000813641.1
• Coordinates: 66446..66664 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFS07_RS00395	61733..62114	+	382	Protein_75	YgiW/YdeI family stress tolerance OB fold protein	-
HFS07_RS00400	62283..62727	+	445	Protein_76	site-specific integrase	-
HFS07_RS00405	62986..63975	-	990	WP_001527061.1	RepB family plasmid replication initiator protein	-
HFS07_RS00410	64469..64765	-	297	WP_001687482.1	hypothetical protein	-
HFS07_RS00415	65249..65770	-	522	WP_010999942.1	hypothetical protein	-
HFS07_RS00420	66446..66664	+	219	WP_000813641.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HFS07_RS00425	66666..66971	+	306	WP_001159863.1	type II toxin-antitoxin system toxin CcdB	Toxin
HFS07_RS00430	66973..67263	+	291	WP_001266176.1	hypothetical protein	-
HFS07_RS00435	67260..67781	+	522	WP_000198608.1	hypothetical protein	-
HFS07_RS00440	67816..68598	+	783	WP_000082169.1	site-specific integrase	-
HFS07_RS00445	68607..69320	+	714	Protein_85	EAL domain-containing protein	-
HFS07_RS00450	69324..69833	+	510	WP_001541544.1	CaiF/GrlA family transcriptional regulator	-
HFS07_RS00455	69827..70312	+	486	WP_000905606.1	membrane protein	-
HFS07_RS00460	70589..70876	-	288	WP_071530243.1	hypothetical protein	-
HFS07_RS00465	71032..71592	+	561	WP_000900095.1	inverse autotransporter beta domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94046	94046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Antitoxin

• Toxin YoeB (5-84)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T154035 WP_001159863.1 NZ_CP051377:66666-66971 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T154035 NZ_CP067303:c2600261-2600010 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT154035 WP_000813641.1 NZ_CP051377:66446-66664 [Salmonella enterica subsp. enterica serovar Typhimurium]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT154035 NZ_CP067303:c2600013-2599759 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: I3W3D5

Antitoxin

• AlphaFold DB: A0A656ICA6