Detailed information of TA system TA154014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1816637..1817227
• Replicon: chromosome
• Accession: NZ_CP051368
• Organism: Salmonella enterica subsp. houtenae serovar 43:z4 strain CVM 24399

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: V0VE57
• Locus tag: HFS24_RS08535
• Protein ID: WP_000373724.1
• Coordinates: 1816895..1817227 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HFS24_RS08530
• Protein ID: WP_079816675.1
• Coordinates: 1816637..1816894 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFS24_RS08505 (1812980)	1812980..1814446	-	1467	WP_002953833.1	C69 family dipeptidase	-
HFS24_RS08510 (1814938)	1814938..1815144	+	207	WP_219827291.1	hypothetical protein	-
HFS24_RS08515 (1815338)	1815338..1815604	-	267	WP_125873103.1	bacteriocin	-
HFS24_RS08520 (1815893)	1815893..1816086	+	194	Protein_1679	hypothetical protein	-
HFS24_RS08525 (1816083)	1816083..1816289	+	207	WP_219827292.1	helix-turn-helix transcriptional regulator	-
HFS24_RS08530 (1816637)	1816637..1816894	+	258	WP_079816675.1	antitoxin	Antitoxin
HFS24_RS08535 (1816895)	1816895..1817227	+	333	WP_000373724.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HFS24_RS08540 (1817837)	1817837..1818622	+	786	Protein_1683	integrase domain-containing protein	-
HFS24_RS08545 (1819148)	1819148..1820397	-	1250	Protein_1684	tyrosine-type recombinase/integrase	-
HFS24_RS08555 (1820990)	1820990..1822089	+	1100	WP_102025837.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	wza / gmd / wcaG / manB / galF / gmd / wcaG / manB / gnd / ugd / hcp2/tssD2	1701119..1835991	134872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11724.57 Da
• Isoelectric Point: 10.5834

>T154014 WP_000373724.1 NZ_CP051368:1816895-1817227 [Salmonella enterica subsp. houtenae serovar 43:z4]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T154014 NZ_CP067299:c3684646-3684350 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9342.80 Da
• Isoelectric Point: 4.4354

>AT154014 WP_079816675.1 NZ_CP051368:1816637-1816894 [Salmonella enterica subsp. houtenae serovar 43:z4]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSDVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT154014 NZ_CP067299:c3684348-3683953 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: V0VE57