Detailed information of TA system TA153992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 74767..75292
• Replicon: plasmid p35161-2
• Accession: NZ_CP051312
• Organism: Salmonella enterica subsp. enterica serovar Heidelberg strain CVM 35161

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: HFU19_RS25175
• Protein ID: WP_001159871.1
• Coordinates: 74987..75292 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: H9TJP1
• Locus tag: HFU19_RS25170
• Protein ID: WP_000813630.1
• Coordinates: 74767..74985 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HFU19_RS25140	69768..70358	-	591	WP_000194550.1	hypothetical protein	-
HFU19_RS25145	70358..70612	-	255	WP_000343103.1	hypothetical protein	-
HFU19_RS25150	70964..71965	+	1002	WP_000774875.1	DUF4238 domain-containing protein	-
HFU19_RS25155	72141..72485	-	345	WP_000793307.1	hypothetical protein	-
HFU19_RS25160	72984..73238	-	255	WP_000678527.1	hypothetical protein	-
HFU19_RS25165	73283..74209	-	927	WP_001290416.1	hypothetical protein	-
HFU19_RS25170	74767..74985	+	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HFU19_RS25175	74987..75292	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
HFU19_RS25180	75293..76099	+	807	WP_000016969.1	site-specific integrase	-
HFU19_RS25185	76279..76923	+	645	WP_001144036.1	ParA family protein	-
HFU19_RS25190	77010..77318	+	309	WP_000030199.1	hypothetical protein	-
HFU19_RS25195	77732..78712	+	981	WP_000688514.1	plasmid segregation protein ParM	-
HFU19_RS25200	78705..79121	+	417	WP_001278818.1	plasmid partitioning/stability family protein	-
HFU19_RS25205	79123..79413	-	291	Protein_84	DUF4113 domain-containing protein	-
HFU19_RS25210	79507..79887	+	381	WP_072157534.1	IS66 family insertion sequence hypothetical protein	-
HFU19_RS25215	79884..80231	+	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..101468	101468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T153992 WP_001159871.1 NZ_CP051312:74987-75292 [Salmonella enterica subsp. enterica serovar Heidelberg]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T153992 NZ_CP067265:3932221-3932556 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT153992 WP_000813630.1 NZ_CP051312:74767-74985 [Salmonella enterica subsp. enterica serovar Heidelberg]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT153992 NZ_CP067265:3931973-3932221 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A829CEF5