Detailed information of TA system TA153968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 488126..488822
• Replicon: chromosome
• Accession: NZ_CP051298
• Organism: Alicycliphilus denitrificans strain DP3

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: HF896_RS02520
• Protein ID: WP_168727636.1
• Coordinates: 488126..488506 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: HF896_RS02525
• Protein ID: WP_168727637.1
• Coordinates: 488487..488822 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF896_RS02510	483879..485573	-	1695	WP_168727634.1	hypothetical protein	-
HF896_RS02515	486738..488045	+	1308	WP_168727635.1	integrase arm-type DNA-binding domain-containing protein	-
HF896_RS02520	488126..488506	+	381	WP_168727636.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF896_RS02525	488487..488822	+	336	WP_168727637.1	helix-turn-helix domain-containing protein	Antitoxin
HF896_RS02530	488809..489120	-	312	WP_168727638.1	AlpA family phage regulatory protein	-
HF896_RS02535	489544..489690	-	147	WP_168727639.1	hypothetical protein	-
HF896_RS02540	489689..490012	+	324	WP_168727640.1	ATPase	-
HF896_RS02545	490155..490523	-	369	WP_168727641.1	DUF2513 domain-containing protein	-
HF896_RS02550	490495..491514	-	1020	WP_168727642.1	reverse transcriptase family protein	-
HF896_RS02555	491480..491785	-	306	WP_168727643.1	helix-turn-helix transcriptional regulator	-
HF896_RS02560	491838..492077	-	240	WP_168727644.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pilT	446741..617649	170908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (17-110)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-96)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14049.16 Da
• Isoelectric Point: 9.6203

>T153968 WP_168727636.1 NZ_CP051298:488126-488506 [Alicycliphilus denitrificans]
MSSTDERPLLWLGSSKKDLLDLPVPVRRFFGHALDLAQRGEQHMGAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVRFREA
VFVLHVFQKKSKRGIATPQADMEIIRQRLKAAAIVAQELRDEEADR

Nucleotide

• Length: 381 bp

>T153968 NZ_CP067261:1966590-1966692 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12125.12 Da
• Isoelectric Point: 9.0499

>AT153968 WP_168727637.1 NZ_CP051298:488487-488822 [Alicycliphilus denitrificans]
MKKRIVEGVEVEASSGNVFADLGLPDADKLKIKSGLTAEIAKAIRVRGLTQAEAAKRMGLTQPKVSALLHGEFSNFSERK
LMDCLNRLGYDIEIKVHPSDQLVGHLMLTHA

Nucleotide

• Length: 336 bp

>AT153968 NZ_CP067261:c1966698-1966554 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT