Detailed information of TA system TA153967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1326702..1327335
• Replicon: chromosome
• Accession: NZ_CP051296
• Organism: Ralstonia solanacearum strain CIAT_078

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F6FXL1
• Locus tag: G8D25_RS14055
• Protein ID: WP_014615899.1
• Coordinates: 1326922..1327335 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F6FXL0
• Locus tag: G8D25_RS14050
• Protein ID: WP_003263271.1
• Coordinates: 1326702..1326935 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G8D25_RS14035 (G8D25_14030)	1322278..1323567	-	1290	WP_003263268.1	MFS transporter	-
G8D25_RS14040 (G8D25_14035)	1323575..1324843	-	1269	WP_124994693.1	AGE family epimerase/isomerase	-
G8D25_RS14045 (G8D25_14040)	1325037..1326644	+	1608	WP_039567295.1	glucan biosynthesis protein	-
G8D25_RS14050 (G8D25_14045)	1326702..1326935	+	234	WP_003263271.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G8D25_RS14055 (G8D25_14050)	1326922..1327335	+	414	WP_014615899.1	PIN domain-containing protein	Toxin
G8D25_RS14060 (G8D25_14055)	1327310..1327936	-	627	WP_014615900.1	DUF1415 family protein	-
G8D25_RS14065 (G8D25_14060)	1328147..1328521	+	375	WP_014615901.1	hypothetical protein	-
G8D25_RS14070 (G8D25_14065)	1328596..1330092	-	1497	WP_014615902.1	glycerol kinase GlpK	-
G8D25_RS14075 (G8D25_14070)	1330264..1332000	-	1737	WP_003266149.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-40)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14919.23 Da
• Isoelectric Point: 5.9701

>T153967 WP_014615899.1 NZ_CP051296:1326922-1327335 [Ralstonia solanacearum]
MPAAEAFFDSNIVLYLLSADAAKADAAEALLMAGGVVSVQVLNETTHVMRRKLAMPWHAIEAVQEAVRAQCRVEPLTVET
HDLGRHLAQRYGLSVYDALIVAAASLAGCRVLYSEDMQHGLVIEQRLRVVNPFFASA

Nucleotide

• Length: 414 bp

>T153967 NZ_CP067261:1799094-1799426 [Klebsiella variicola]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTCCTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACTCGGCTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGCTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGAACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCAGACGCCGTTGTCAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTCAGTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8664.82 Da
• Isoelectric Point: 8.7288

>AT153967 WP_003263271.1 NZ_CP051296:1326702-1326935 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALGLHEGDDVEVVVAGERSFGVRRKRSARELFERLHQYRGKLPADFIFERDDANARG

Nucleotide

• Length: 234 bp

>AT153967 NZ_CP067261:1798836-1799093 [Klebsiella variicola]
ATGTACAAAACTCGCCTGAAAAAGGTTGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAGCGCTGGAGCT
GTCGACAGACAGCGAAGTGGGTATGACTATAGATAATGGCTGCCTGATTATTGAACCGCAGAAACGGCCCCATTATTCGC
TTGAGGAACTGTTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Structures

Toxin

• AlphaFold DB: F6FXL1

Antitoxin

• AlphaFold DB: F6FXL0