Detailed information of TA system TA153964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 95663..96264
• Replicon: plasmid pMSNZP2077A
• Accession: NZ_CP051294
• Organism: Mesorhizobium sp. NZP2077

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A6M7Y451
• Locus tag: HGP13_RS36305
• Protein ID: WP_027054595.1
• Coordinates: 95941..96264 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: HGP13_RS36300
• Protein ID: WP_172235236.1
• Coordinates: 95663..95944 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGP13_RS36275	91025..91297	-	273	WP_172235059.1	WGR domain-containing protein	-
HGP13_RS36280	91512..91853	-	342	WP_172235060.1	hypothetical protein	-
HGP13_RS36285	91850..92542	-	693	WP_172235061.1	ParA family protein	-
HGP13_RS36290	92962..93279	+	318	WP_172235235.1	hypothetical protein	-
HGP13_RS36295	93276..95546	+	2271	WP_172235062.1	relaxase/mobilization nuclease domain-containing protein	-
HGP13_RS36300	95663..95944	-	282	WP_172235236.1	XRE family transcriptional regulator	Antitoxin
HGP13_RS36305	95941..96264	-	324	WP_027054595.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HGP13_RS36310	96485..98809	-	2325	WP_172235063.1	type IV secretory system conjugative DNA transfer family protein	-
HGP13_RS36315	98787..99833	-	1047	WP_172235064.1	P-type DNA transfer ATPase VirB11	-
HGP13_RS36320	99799..101136	-	1338	WP_120018525.1	type IV secretion system protein VirB10	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..364822	364822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-101)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-90)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12289.12 Da
• Isoelectric Point: 10.1375

>T153964 WP_027054595.1 NZ_CP051294:c96264-95941 [Mesorhizobium sp. NZP2077]
MKVIEWLGSSRADVRAFPDDARIEAGWQLELVQRGDDPDDWKPMQIVGAGVREIRVREASGAFRVIYLATLEDRVLVLHA
FQKKTQATSKKDLDLAAQRLKRWKAER

Nucleotide

• Length: 324 bp

>T153964 NZ_CP067261:326620-326988 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGACGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGCCATATTTTCAATGATGGAAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10483.13 Da
• Isoelectric Point: 10.0424

>AT153964 WP_172235236.1 NZ_CP051294:c95944-95663 [Mesorhizobium sp. NZP2077]
MNVEIYENVWDALADTKQEAANLKARSALLYEIRKAVQRWDIPQEEAAKRLGLTRPRTNDLLRGKLAKFSLDALVNIAAS
AHLVIEIRVKQAA

Nucleotide

• Length: 282 bp

>AT153964 NZ_CP067261:326402-326623 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A6M7Y451