Detailed information of TA system TA153950

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4934572..4935407
• Replicon: chromosome
• Accession: NZ_CP051263
• Organism: Escherichia coli CFT073

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VR61
• Locus tag: HGG68_RS23620
• Protein ID: WP_000854747.1
• Coordinates: 4934572..4934949 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HGG68_RS23625
• Protein ID: WP_024182258.1
• Coordinates: 4935039..4935407 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGG68_RS23590	4930523..4930864	-	342	Protein_4608	DUF4942 domain-containing protein	-
HGG68_RS23595	4930958..4932457	+	1500	WP_000174402.1	IS21-like element ISEc10 family transposase	-
HGG68_RS23600	4932454..4933209	+	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
HGG68_RS23605	4933275..4933793	-	519	Protein_4611	DUF4942 domain-containing protein	-
HGG68_RS23610	4933878..4934075	-	198	WP_000839291.1	DUF957 domain-containing protein	-
HGG68_RS23615	4934087..4934575	-	489	WP_000761667.1	hypothetical protein	-
HGG68_RS23620	4934572..4934949	-	378	WP_000854747.1	TA system toxin CbtA family protein	Toxin
HGG68_RS23625	4935039..4935407	-	369	WP_024182258.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HGG68_RS23630	4935486..4935707	-	222	WP_000692312.1	DUF987 domain-containing protein	-
HGG68_RS23635	4935776..4936252	-	477	WP_001186756.1	RadC family protein	-
HGG68_RS23640	4936267..4936752	-	486	WP_000860054.1	antirestriction protein	-
HGG68_RS23645	4936843..4937661	-	819	WP_001175140.1	DUF945 domain-containing protein	-
HGG68_RS23650	4937751..4937984	-	234	WP_001278280.1	DUF905 family protein	-
HGG68_RS23655	4937990..4938667	-	678	WP_001097307.1	hypothetical protein	-
HGG68_RS23660	4938815..4939495	-	681	WP_001282928.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14095.03 Da
• Isoelectric Point: 6.8524

>T153950 WP_000854747.1 NZ_CP051263:c4934949-4934572 [Escherichia coli CFT073]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNDITLGKHPEAKQ

Nucleotide

• Length: 378 bp

>T153950 NZ_CP067255:349413-349760 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13497.32 Da
• Isoelectric Point: 6.7344

>AT153950 WP_024182258.1 NZ_CP051263:c4935407-4935039 [Escherichia coli CFT073]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLGQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGCVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT153950 NZ_CP067255:349760-350059 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H2VFZ1