Detailed information of TA system TA153920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3756839..3757538
• Replicon: chromosome
• Accession: NZ_CP051260
• Organism: Proteus mirabilis strain STP3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: HF524_RS17540
• Protein ID: WP_004249093.1
• Coordinates: 3756839..3757225 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: HF524_RS17545
• Protein ID: WP_004246828.1
• Coordinates: 3757218..3757538 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF524_RS17525	3752858..3753439	-	582	WP_004246833.1	DNA-3-methyladenine glycosylase I	-
HF524_RS17530	3753690..3754595	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
HF524_RS17535	3754605..3756677	+	2073	WP_041701373.1	glycine--tRNA ligase subunit beta	-
HF524_RS17540	3756839..3757225	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF524_RS17545	3757218..3757538	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
HF524_RS17550	3757587..3758021	-	435	WP_004246827.1	hypothetical protein	-
HF524_RS17555	3758014..3758898	-	885	WP_110706881.1	endonuclease/exonuclease/phosphatase family protein	-
HF524_RS17560	3759115..3760401	-	1287	WP_049256516.1	DUF3748 domain-containing protein	-
HF524_RS17565	3760538..3761155	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
HF524_RS17570	3761457..3762080	+	624	WP_004246821.1	guanylate kinase	-
HF524_RS17575	3762135..3762410	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T153920 WP_004249093.1 NZ_CP051260:3756839-3757225 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T153920 NZ_CP067245:3809488-3809784 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT153920 WP_004246828.1 NZ_CP051260:3757218-3757538 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT153920 NZ_CP067245:3809786-3810181 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97