Detailed information of TA system TA153916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 978080..978731
• Replicon: chromosome
• Accession: NZ_CP051227
• Organism: Lacticaseibacillus rhamnosus strain LDTM7511

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: HF522_RS04645
• Protein ID: WP_005686631.1
• Coordinates: 978348..978731 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: HF522_RS04640
• Protein ID: WP_005686632.1
• Coordinates: 978080..978328 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF522_RS04620 (HF522_04850)	973129..974517	+	1389	WP_015765066.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
HF522_RS04625 (HF522_04855)	974791..976299	+	1509	WP_005686635.1	DEAD/DEAH box helicase	-
HF522_RS04630 (HF522_04860)	976468..976842	+	375	WP_005686634.1	holo-ACP synthase	-
HF522_RS04635 (HF522_04865)	976829..977968	+	1140	WP_005686633.1	alanine racemase	-
HF522_RS04640 (HF522_04870)	978080..978328	+	249	WP_005686632.1	antitoxin	Antitoxin
HF522_RS04645 (HF522_04875)	978348..978731	+	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HF522_RS04655 (HF522_04890)	979168..979695	+	528	WP_005686630.1	QueT transporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T153916 WP_005686631.1 NZ_CP051227:978348-978731 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T153916 NZ_CP067245:c2607182-2607009 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT153916 WP_005686632.1 NZ_CP051227:978080-978328 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT153916 NZ_CP067245:c2607506-2607210 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATATTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19