Detailed information of TA system TA153908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 105391..106016
• Replicon: plasmid pSCZE2
• Accession: NZ_CP051224
• Organism: Escherichia coli strain SCZE5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HF562_RS24000
• Protein ID: WP_000911313.1
• Coordinates: 105618..106016 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: HF562_RS23995
• Protein ID: WP_000450520.1
• Coordinates: 105391..105618 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF562_RS23995	105391..105618	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HF562_RS24000	105618..106016	+	399	WP_000911313.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HF562_RS24005	106025..107071	-	1047	Protein_115	type IV secretion system DNA-binding domain-containing protein	-
HF562_RS24010	107360..107581	-	222	WP_001278689.1	conjugal transfer protein TraR	-
HF562_RS24015	107716..108231	-	516	WP_000809838.1	type IV conjugative transfer system lipoprotein TraV	-
HF562_RS24020	108228..108479	-	252	WP_001038342.1	conjugal transfer protein TrbG	-
HF562_RS24025	108491..108721	-	231	WP_001352845.1	conjugal transfer protein TrbD	-
HF562_RS24030	108675..109265	-	591	WP_001376242.1	conjugal transfer protein TraP	-
HF562_RS24035	109255..110682	-	1428	WP_000146685.1	conjugal transfer protein TraB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qacE / aadA2 / dfrA12 / oqxB / oqxA / mph(A) / aac(3)-IId / blaTEM-1A / floR / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..143259	143259

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T153908 WP_000911313.1 NZ_CP051224:105618-106016 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T153908 NZ_CP067245:312295-312398 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT153908 WP_000450520.1 NZ_CP051224:105391-105618 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT153908 NZ_CP067245:c312436-312165 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: V0VCB2