Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3571340..3571958 Replicon chromosome
Accession NZ_CP051222
Organism Escherichia coli strain SCZE5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HF562_RS17380 Protein ID WP_001291435.1
Coordinates 3571740..3571958 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HF562_RS17375 Protein ID WP_000344800.1
Coordinates 3571340..3571714 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF562_RS17365 3566429..3567622 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HF562_RS17370 3567645..3570794 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HF562_RS17375 3571340..3571714 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HF562_RS17380 3571740..3571958 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HF562_RS17385 3572130..3572681 + 552 WP_000102564.1 maltose O-acetyltransferase -
HF562_RS17390 3572797..3573267 + 471 WP_000136192.1 YlaC family protein -
HF562_RS17395 3573431..3574981 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HF562_RS17400 3575023..3575376 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HF562_RS17410 3575755..3576066 + 312 WP_000409911.1 MGMT family protein -
HF562_RS17415 3576097..3576669 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T153899 WP_001291435.1 NZ_CP051222:3571740-3571958 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T153899 NZ_CP067241:c4332767-4332477 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACTGGGATATCGCCTGGTCTATTCCGTCAATGATAAAGAGTTACTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAAAACTCAGCCGTTTATGAAGACGCGACAAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT153899 WP_000344800.1 NZ_CP051222:3571340-3571714 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT153899 NZ_CP067241:c4332999-4332757 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATTACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References