Detailed information of TA system TA153896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2550076..2550714
• Replicon: chromosome
• Accession: NZ_CP051222
• Organism: Escherichia coli strain SCZE5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HF562_RS12445
• Protein ID: WP_000813794.1
• Coordinates: 2550538..2550714 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HF562_RS12440
• Protein ID: WP_001270286.1
• Coordinates: 2550076..2550492 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF562_RS12420	2545228..2546169	-	942	WP_001251313.1	ABC transporter permease	-
HF562_RS12425	2546170..2547183	-	1014	WP_000220415.1	ABC transporter ATP-binding protein	-
HF562_RS12430	2547201..2548346	-	1146	WP_113403949.1	ABC transporter substrate-binding protein	-
HF562_RS12435	2548591..2549997	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
HF562_RS12440	2550076..2550492	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HF562_RS12445	2550538..2550714	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HF562_RS12450	2550936..2551166	+	231	WP_000494244.1	YncJ family protein	-
HF562_RS12455	2551258..2553219	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HF562_RS12460	2553292..2553828	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
HF562_RS12465	2553881..2555095	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2546170..2570546	24376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T153896 WP_000813794.1 NZ_CP051222:c2550714-2550538 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T153896 NZ_CP067241:3706830-3707048 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT153896 WP_001270286.1 NZ_CP051222:c2550492-2550076 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT153896 NZ_CP067241:3706430-3706804 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• PDB: 6HPB