Detailed information of TA system TA153895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2407310..2407836
• Replicon: chromosome
• Accession: NZ_CP051222
• Organism: Escherichia coli strain SCZE5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HF562_RS11725
• Protein ID: WP_000323025.1
• Coordinates: 2407549..2407836 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: HF562_RS11720
• Protein ID: WP_000534858.1
• Coordinates: 2407310..2407549 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF562_RS11700	2403150..2404519	+	1370	WP_168728556.1	IS3-like element IS150 family transposase	-
HF562_RS11705	2404695..2406008	+	1314	WP_000589005.1	ISNCY family transposase	-
HF562_RS11710	2406445..2406777	-	333	WP_001301033.1	protein FlxA	-
HF562_RS11715	2406980..2407285	-	306	WP_071598992.1	hypothetical protein	-
HF562_RS11720	2407310..2407549	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HF562_RS11725	2407549..2407836	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HF562_RS11730	2407908..2408063	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
HF562_RS11735	2408280..2408531	+	252	WP_000980994.1	hypothetical protein	-
HF562_RS11740	2408757..2408918	+	162	Protein_2297	antitermination protein	-
HF562_RS11745	2409196..2409285	-	90	WP_120795389.1	hypothetical protein	-
HF562_RS11750	2409340..2409552	-	213	WP_000087756.1	cold shock-like protein CspF	-
HF562_RS11755	2409853..2410068	+	216	WP_000066484.1	cold shock-like protein CspB	-
HF562_RS11760	2410822..2411037	+	216	WP_000839590.1	phage lysis protein EssD	-
HF562_RS11765	2411042..2411353	+	312	WP_000189916.1	YdfR family protein	-
HF562_RS11770	2411350..2411883	+	534	WP_001092971.1	lysozyme	-
HF562_RS11775	2411880..2412377	+	498	WP_001071769.1	DUF2514 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2381134..2425167	44033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T153895 WP_000323025.1 NZ_CP051222:2407549-2407836 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T153895 NZ_CP067241:c3679032-3678730 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT153895 WP_000534858.1 NZ_CP051222:2407310-2407549 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT153895 NZ_CP067241:c3678694-3678353 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8