Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1138626..1139209 Replicon chromosome
Accession NZ_CP051222
Organism Escherichia coli strain SCZE5

Toxin (Protein)


Gene name mazF Uniprot ID A0A1V3W215
Locus tag HF562_RS05495 Protein ID WP_000254741.1
Coordinates 1138874..1139209 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag HF562_RS05490 Protein ID WP_000581937.1
Coordinates 1138626..1138874 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF562_RS05480 1134965..1136266 + 1302 WP_168728538.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
HF562_RS05485 1136314..1138548 + 2235 WP_000226815.1 GTP pyrophosphokinase -
HF562_RS05490 1138626..1138874 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
HF562_RS05495 1138874..1139209 + 336 WP_000254741.1 endoribonuclease MazF Toxin
HF562_RS05500 1139280..1140071 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
HF562_RS05505 1140299..1141936 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
HF562_RS05510 1142024..1143322 + 1299 WP_000036723.1 phosphopyruvate hydratase -
HF562_RS05515 1143382..1143852 - 471 WP_113403894.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12114.04 Da        Isoelectric Point: 8.2618

>T153885 WP_000254741.1 NZ_CP051222:1138874-1139209 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGSTKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T153885 NZ_CP067241:c921603-921226 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT153885 WP_000581937.1 NZ_CP051222:1138626-1138874 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT153885 NZ_CP067241:c922061-921693 [Escherichia coli]
GTGTCAGACTCGCGCCATGAGACAAATTATCCCGACGACCATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
ACCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1V3W215

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References