Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1025824..1026478 Replicon chromosome
Accession NZ_CP051222
Organism Escherichia coli strain SCZE5

Toxin (Protein)


Gene name cptA Uniprot ID A0A140N853
Locus tag HF562_RS05000 Protein ID WP_000244783.1
Coordinates 1026071..1026478 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HF562_RS04995 Protein ID WP_000354046.1
Coordinates 1025824..1026090 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF562_RS04970 1020993..1021736 + 744 WP_000951948.1 SDR family oxidoreductase -
HF562_RS04975 1021793..1023226 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
HF562_RS04980 1023271..1023582 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HF562_RS04985 1023746..1024405 + 660 WP_000250274.1 hemolysin III family protein -
HF562_RS04990 1024601..1025581 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HF562_RS04995 1025824..1026090 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HF562_RS05000 1026071..1026478 + 408 WP_000244783.1 protein YgfX Toxin
HF562_RS05005 1026518..1027039 - 522 WP_001055874.1 flavodoxin FldB -
HF562_RS05010 1027151..1028047 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HF562_RS05015 1028072..1028782 + 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HF562_RS05020 1028788..1030521 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15962.85 Da        Isoelectric Point: 11.2669

>T153884 WP_000244783.1 NZ_CP051222:1026071-1026478 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEISLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T153884 NZ_CP067241:c885889-885515 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT153884 WP_000354046.1 NZ_CP051222:1025824-1026090 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT153884 NZ_CP067241:c886347-885979 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140N853

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References