Detailed information of TA system TA153882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 895059..895837
• Replicon: chromosome
• Accession: NZ_CP051222
• Organism: Escherichia coli strain SCZE5

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HF562_RS04280
• Protein ID: WP_000622309.1
• Coordinates: 895349..895837 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7H9KCB5
• Locus tag: HF562_RS04275
• Protein ID: WP_001110459.1
• Coordinates: 895059..895352 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF562_RS04255	890823..891806	-	984	WP_162753665.1	KpsF/GutQ family sugar-phosphate isomerase	-
HF562_RS04260	892631..892966	-	336	WP_000789899.1	hypothetical protein	-
HF562_RS04265	892963..893436	-	474	WP_000143249.1	hypothetical protein	-
HF562_RS04270	894029..894525	+	497	Protein_840	type VI secretion system contractile sheath small subunit	-
HF562_RS04275	895059..895352	+	294	WP_001110459.1	DUF1778 domain-containing protein	Antitoxin
HF562_RS04280	895349..895837	+	489	WP_000622309.1	GNAT family N-acetyltransferase	Toxin
HF562_RS04285	896678..897520	-	843	WP_039004748.1	DUF4942 domain-containing protein	-
HF562_RS04290	897605..897802	-	198	WP_023563519.1	DUF957 domain-containing protein	-
HF562_RS04295	897878..898027	-	150	Protein_845	hypothetical protein	-
HF562_RS04300	898024..898401	-	378	WP_057109034.1	TA system toxin CbtA family protein	-
HF562_RS04305	898491..898859	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	-
HF562_RS24860	898933..899154	-	222	Protein_848	DUF987 domain-containing protein	-
HF562_RS04320	899241..899717	-	477	WP_057109035.1	RadC family protein	-
HF562_RS04325	899733..900212	-	480	WP_057109036.1	antirestriction protein	-
HF562_RS04330	900312..900452	+	141	WP_000997937.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (83-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17491.33 Da
• Isoelectric Point: 7.7535

>T153882 WP_000622309.1 NZ_CP051222:895349-895837 [Escherichia coli]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVTGASRTFVCCGSDSKVLAYYSLASSAVTTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSIHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLLVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T153882 NZ_CP067241:c678309-677845 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10949.56 Da
• Isoelectric Point: 9.9171

>AT153882 WP_001110459.1 NZ_CP051222:895059-895352 [Escherichia coli]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQTFLTRLDQAPAPN
SALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT153882 NZ_CP067241:c678644-678309 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7H9KCB5