153873

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 36188..36713 Replicon plasmid pSCZP1
Accession NZ_CP051220
Organism Escherichia coli strain SFE8

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HF527_RS24660 Protein ID WP_001159871.1
Coordinates 36188..36493 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag HF527_RS24665 Protein ID WP_000813639.1
Coordinates 36495..36713 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF527_RS24625 31312..31743 - 432 Protein_34 relaxase NikB -
HF527_RS24630 31850..33011 + 1162 Protein_35 IS3 family transposase -
HF527_RS25475 33129..34099 - 971 Protein_36 plasmid segregation protein parM -
HF527_RS24645 34328..34972 + 645 WP_000633913.1 AAA family ATPase -
HF527_RS24650 34966..35241 + 276 WP_000239529.1 hypothetical protein -
HF527_RS24655 35378..36187 - 810 WP_113452213.1 site-specific integrase -
HF527_RS24660 36188..36493 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HF527_RS24665 36495..36713 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HF527_RS24670 37271..41092 - 3822 WP_106693592.1 hypothetical protein -
HF527_RS24675 41336..41497 + 162 WP_000251882.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - afaC-I 1..107230 107230


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T153873 WP_001159871.1 NZ_CP051220:c36493-36188 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T153873 NZ_CP067236:c72732-72583 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT153873 WP_000813639.1 NZ_CP051220:c36713-36495 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT153873 NZ_CP067236:72776-72837 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References