Detailed information of TA system TA153872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1823..2358
• Replicon: plasmid pSCZP1
• Accession: NZ_CP051220
• Organism: Escherichia coli strain SFE8

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: HF527_RS24470
• Protein ID: WP_000222766.1
• Coordinates: 2071..2358 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HF527_RS24465
• Protein ID: WP_106693621.1
• Coordinates: 1823..2074 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF527_RS24455	1..858	+	858	WP_072834368.1	incFII family plasmid replication initiator RepA	-
HF527_RS24460	1559..1750	+	192	WP_072656942.1	hypothetical protein	-
HF527_RS24465	1823..2074	+	252	WP_106693621.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HF527_RS24470	2071..2358	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF527_RS24475	2528..2932	+	405	WP_000154256.1	DUF2251 domain-containing protein	-
HF527_RS24480	3087..3467	+	381	WP_001171554.1	IS66 family insertion sequence hypothetical protein	-
HF527_RS24485	3464..3811	+	348	WP_000612591.1	IS66 family insertion sequence element accessory protein TnpB	-
HF527_RS24490	3861..5399	+	1539	WP_106693623.1	IS66 family transposase	-
HF527_RS24495	5511..5846	-	336	WP_021531026.1	immunity protein	-
HF527_RS24500	5975..6322	+	348	WP_106693625.1	hypothetical protein	-
HF527_RS24505	6340..6930	-	591	WP_106693627.1	hypothetical protein	-
HF527_RS24510	6927..7166	-	240	WP_168725371.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	afaC-I	1..107230	107230

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T153872 WP_000222766.1 NZ_CP051220:2071-2358 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T153872 NZ_CP067236:c17358-17053 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9201.38 Da
• Isoelectric Point: 4.0780

>AT153872 WP_106693621.1 NZ_CP051220:1823-2074 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQSLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT153872 NZ_CP067236:c17578-17360 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2