Detailed information of TA system TA153870

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4562415..4562912
• Replicon: chromosome
• Accession: NZ_CP051219
• Organism: Escherichia coli strain SFE8

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: HF527_RS22200
• Protein ID: WP_001173343.1
• Coordinates: 4562415..4562588 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: HF527_RS22205
• Protein ID: WP_071988580.1
• Coordinates: 4562616..4562912 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF527_RS22190	4559085..4560542	+	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-
HF527_RS22195	4560779..4562296	+	1518	WP_001295087.1	lysine--tRNA ligase	-
HF527_RS22200	4562415..4562588	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
HF527_RS22205	4562616..4562912	-	297	WP_071988580.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
HF527_RS22210	4563139..4563411	-	273	WP_000405647.1	N-acetyltransferase	-
HF527_RS22215	4563624..4567553	-	3930	WP_046201569.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T153870 WP_001173343.1 NZ_CP051219:c4562588-4562415 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T153870 NZ_CP067235:c4508985-4508767 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11482.00 Da
• Isoelectric Point: 4.1705

>AT153870 WP_071988580.1 NZ_CP051219:c4562912-4562616 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSTIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT153870 NZ_CP067235:c4509385-4509011 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACTTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTCCAAAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41