Detailed information of TA system TA153867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4340514..4341849
• Replicon: chromosome
• Accession: NZ_CP051219
• Organism: Escherichia coli strain SFE8

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: F4VMR9
• Locus tag: HF527_RS21105
• Protein ID: WP_000342497.1
• Coordinates: 4340514..4341461 (-)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A228Z6F8
• Locus tag: HF527_RS21110
• Protein ID: WP_000152739.1
• Coordinates: 4341454..4341849 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF527_RS21075	4336367..4337083	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
HF527_RS21080	4337103..4338209	+	1107	WP_001044128.1	N-acetylneuraminate epimerase	-
HF527_RS21085	4338274..4339254	+	981	WP_001366032.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
HF527_RS21090	4339262..4339912	-	651	WP_001037970.1	HNH endonuclease	-
HF527_RS21100	4340291..4340476	+	186	WP_000066585.1	hypothetical protein	-
HF527_RS21105	4340514..4341461	-	948	WP_000342497.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HF527_RS21110	4341454..4341849	-	396	WP_000152739.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HF527_RS21115	4341919..4342752	-	834	WP_001192741.1	DUF4942 domain-containing protein	-
HF527_RS21120	4343058..4343300	+	243	WP_168725362.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
HF527_RS21125	4343376..4344604	+	1229	WP_102384962.1	IS3 family transposase	-
HF527_RS21130	4344616..4345950	+	1335	Protein_4139	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	4331602..4347113	15511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-303)

Antitoxin

• Protein of unknown function DUF6088 (7-108)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 35588.79 Da
• Isoelectric Point: 4.9737

>T153867 WP_000342497.1 NZ_CP051219:c4341461-4340514 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Nucleotide

• Length: 948 bp

>T153867 NZ_CP067235:3736034-3736384 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14605.90 Da
• Isoelectric Point: 11.6915

>AT153867 WP_000152739.1 NZ_CP051219:c4341849-4341454 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Nucleotide

• Length: 396 bp

>AT153867 NZ_CP067235:3735789-3736040 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A1L5BAQ6

Antitoxin

• AlphaFold DB: A0A228Z6F8