Detailed information of TA system TA153862

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2264869..2265701
• Replicon: chromosome
• Accession: NZ_CP051219
• Organism: Escherichia coli strain SFE8

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0D8W767
• Locus tag: HF527_RS11050
• Protein ID: WP_000854697.1
• Coordinates: 2264869..2265243 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0D8WAR2
• Locus tag: HF527_RS11055
• Protein ID: WP_001354195.1
• Coordinates: 2265333..2265701 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF527_RS11020	2260019..2260672	-	654	WP_001355839.1	ATP-binding cassette domain-containing protein	-
HF527_RS11025	2260672..2262207	-	1536	WP_000258506.1	ABC transporter permease	-
HF527_RS11030	2262180..2263346	-	1167	WP_001215353.1	ABC transporter substrate-binding protein	-
HF527_RS11035	2263566..2264362	-	797	Protein_2157	DUF4942 domain-containing protein	-
HF527_RS11040	2264447..2264644	-	198	WP_085975623.1	DUF957 domain-containing protein	-
HF527_RS11045	2264720..2264872	-	153	Protein_2159	hypothetical protein	-
HF527_RS11050	2264869..2265243	-	375	WP_000854697.1	TA system toxin CbtA family protein	Toxin
HF527_RS11055	2265333..2265701	-	369	WP_001354195.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HF527_RS11060	2265751..2266395	-	645	WP_045146861.1	hypothetical protein	-
HF527_RS11065	2266414..2266635	-	222	WP_000692303.1	DUF987 domain-containing protein	-
HF527_RS11070	2266698..2267174	-	477	WP_001354455.1	RadC family protein	-
HF527_RS11075	2267190..2267669	-	480	WP_000860074.1	antirestriction protein	-
HF527_RS11080	2267751..2268569	-	819	WP_168725331.1	DUF945 domain-containing protein	-
HF527_RS11085	2268670..2268822	-	153	WP_001696589.1	DUF905 family protein	-
HF527_RS11090	2268828..2269505	-	678	WP_001097365.1	hypothetical protein	-
HF527_RS11095	2269624..2270508	-	885	WP_001696588.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14023.07 Da
• Isoelectric Point: 7.1881

>T153862 WP_000854697.1 NZ_CP051219:c2265243-2264869 [Escherichia coli]
MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFADERVIELHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T153862 NZ_CP067235:c2874190-2874083 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTCGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13708.48 Da
• Isoelectric Point: 6.8270

>AT153862 WP_001354195.1 NZ_CP051219:c2265701-2265333 [Escherichia coli]
VSDTLPGTTHHDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYHPDQAFPLPMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT153862 NZ_CP067235:2874247-2874302 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Toxin

• AlphaFold DB: A0A0D8W767

Antitoxin

• AlphaFold DB: A0A0D8WAR2