153852

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1435961..1436586 Replicon chromosome
Accession NZ_CP051219
Organism Escherichia coli strain SFE8

Toxin (Protein)


Gene name vapC Uniprot ID A0A2J1DF47
Locus tag HF527_RS07050 Protein ID WP_000911331.1
Coordinates 1436188..1436586 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HF527_RS07045 Protein ID WP_000450524.1
Coordinates 1435961..1436188 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF527_RS07020 1431764..1432234 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HF527_RS07025 1432234..1432806 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HF527_RS07030 1432952..1433830 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HF527_RS07035 1433847..1434881 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
HF527_RS07040 1435094..1435807 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HF527_RS07045 1435961..1436188 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HF527_RS07050 1436188..1436586 + 399 WP_000911331.1 tRNA(fMet)-specific endonuclease VapC Toxin
HF527_RS07055 1436733..1437596 + 864 WP_001267507.1 neutral zinc metallopeptidase -
HF527_RS07060 1437611..1439626 + 2016 WP_001543081.1 tRNA cytosine(34) acetyltransferase TmcA -
HF527_RS07065 1439700..1440398 + 699 WP_000679812.1 esterase -
HF527_RS07070 1440508..1440708 - 201 WP_089631931.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14862.19 Da        Isoelectric Point: 9.2216

>T153852 WP_000911331.1 NZ_CP051219:1436188-1436586 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLVELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T153852 NZ_CP067233:59089-59241 [Escherichia coli]
ATGCCACAGCGAACATTTTTAATGATGTTGATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGGCTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT153852 WP_000450524.1 NZ_CP051219:1435961-1436188 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT153852 NZ_CP067233:c59039-58977 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J1DF47

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References