Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 955478..956132 Replicon chromosome
Accession NZ_CP051219
Organism Escherichia coli strain SFE8

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HF527_RS04665 Protein ID WP_000244772.1
Coordinates 955725..956132 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HF527_RS04660 Protein ID WP_000354046.1
Coordinates 955478..955744 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HF527_RS04635 950647..951390 + 744 WP_000951964.1 SDR family oxidoreductase -
HF527_RS04640 951447..952880 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
HF527_RS04645 952925..953236 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
HF527_RS04650 953400..954059 + 660 WP_000250274.1 hemolysin III family protein -
HF527_RS04655 954255..955235 - 981 WP_000886095.1 tRNA-modifying protein YgfZ -
HF527_RS04660 955478..955744 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HF527_RS04665 955725..956132 + 408 WP_000244772.1 protein YgfX Toxin
HF527_RS04670 956172..956693 - 522 WP_001055874.1 flavodoxin FldB -
HF527_RS04675 956805..957701 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HF527_RS04680 957726..958436 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HF527_RS04685 958442..960175 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T153850 WP_000244772.1 NZ_CP051219:955725-956132 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T153850 NZ_CP067232:4257350-4257457 [Escherichia coli]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT153850 WP_000354046.1 NZ_CP051219:955478-955744 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT153850 NZ_CP067232:c4257297-4257236 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References