Detailed information of TA system TA153845

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4781127..4781743
• Replicon: chromosome
• Accession: NZ_CP051218
• Organism: Salmonella sp. SCFS4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8Z2U3
• Locus tag: HF553_RS22990
• Protein ID: WP_000238494.1
• Coordinates: 4781127..4781501 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8E9YHE9
• Locus tag: HF553_RS22995
• Protein ID: WP_001523745.1
• Coordinates: 4781501..4781743 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF553_RS22975	4778629..4779531	+	903	WP_051129234.1	formate dehydrogenase subunit beta	-
HF553_RS22980	4779528..4780163	+	636	WP_000829028.1	formate dehydrogenase cytochrome b556 subunit	-
HF553_RS22985	4780160..4781089	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
HF553_RS22990	4781127..4781501	-	375	WP_000238494.1	type II toxin-antitoxin system VapC family toxin	Toxin
HF553_RS22995	4781501..4781743	-	243	WP_001523745.1	ribbon-helix-helix domain-containing protein	Antitoxin
HF553_RS23000	4781948..4782877	+	930	WP_001162861.1	alpha/beta hydrolase	-
HF553_RS23005	4782963..4783274	+	312	WP_000558165.1	type II toxin-antitoxin system HigB family toxin	-
HF553_RS23010	4783271..4783717	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
HF553_RS23015	4783732..4784673	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
HF553_RS23020	4784718..4785155	-	438	WP_000560969.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HF553_RS23025	4785152..4786024	-	873	WP_000921423.1	virulence factor BrkB family protein	-
HF553_RS23030	4786018..4786617	-	600	WP_023227076.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13908.18 Da
• Isoelectric Point: 7.3567

>T153845 WP_000238494.1 NZ_CP051218:c4781501-4781127 [Salmonella sp. SCFS4]
MVKGSALFDTNILIDLFSGRIEAKHALEAYPPQNAISLITWMEVMVGAKKYHQENRTRIALSAFNIIGVTQEIAERSVIV
RQEYGMKLPDAIILATAQVHRCELVTRNTKDFADIPGVITPYHL

Nucleotide

• Length: 375 bp

>T153845 NZ_CP067232:3500317-3500571 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9437.77 Da
• Isoelectric Point: 4.4463

>AT153845 WP_001523745.1 NZ_CP051218:c4781743-4781501 [Salmonella sp. SCFS4]
MMAGMDMGRILLDLSDDVIKRLDDLKVQRNLPRAELLREAVEQYLERQDRAETTISKALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT153845 NZ_CP067232:3500069-3500320 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A5I6CND8