Detailed information of TA system TA153789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/DUF433(antitoxin)
• Location: 19352..19923
• Replicon: chromosome
• Accession: NZ_CP051206
• Organism: Dolichospermum flos-aquae CCAP 1403/13F

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HGD76_RS00100
• Protein ID: WP_148759831.1
• Coordinates: 19582..19923 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HGD76_RS00095
• Protein ID: WP_148759833.1
• Coordinates: 19352..19585 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HGD76_RS00060	14873..15298	-	426	WP_015078218.1	50S ribosomal protein L11	-
HGD76_RS00065	15305..15937	-	633	WP_015078219.1	transcription termination/antitermination protein NusG	-
HGD76_RS00070	15937..16158	-	222	WP_015078220.1	preprotein translocase subunit SecE	-
HGD76_RS00080	16566..16928	-	363	WP_015078221.1	50S ribosomal protein L19	-
HGD76_RS00085	17268..19004	+	1737	WP_148759835.1	GTPase HflX	-
HGD76_RS00090	19131..19331	-	201	Protein_16	hypothetical protein	-
HGD76_RS00095	19352..19585	+	234	WP_148759833.1	DUF433 domain-containing protein	Antitoxin
HGD76_RS00100	19582..19923	+	342	WP_148759831.1	DUF5615 family PIN-like protein	Toxin
HGD76_RS00105	20406..22049	-	1644	WP_168694572.1	hypothetical protein	-
HGD76_RS00110	22249..23166	-	918	WP_168694573.1	hypothetical protein	-
HGD76_RS00115	23345..23593	+	249	WP_039203265.1	hypothetical protein	-
HGD76_RS00120	23583..23957	+	375	WP_210967685.1	putative toxin-antitoxin system toxin component, PIN family	-
HGD76_RS00125	24037..24267	+	231	WP_137908734.1	hypothetical protein	-
HGD76_RS00130	24257..24607	+	351	WP_168694574.1	type II toxin-antitoxin system PemK/MazF family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Domain of unknown function DUF5615 (1-78)

Antitoxin

• Protein of unknown function DUF433 (11-66)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12737.92 Da
• Isoelectric Point: 4.3063

>T153789 WP_148759831.1 NZ_CP051206:19582-19923 [Dolichospermum flos-aquae CCAP 1403/13F]
MKIWIDAQLPATLAIWVTDTFAIEAFSLRDIGLRDAKDIEIFEAAKIANVIIMTKDSDFVDLVCRLGIPPQILWLTCGNV
TNRNLRQLLIITLPDALEQLQQGEMIVEISNSR

Nucleotide

• Length: 342 bp

>T153789 NZ_CP067093:c5513242-5512985 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACGTTCGCGATACATC
AGGCCGCGTCACCTCCGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAACAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8662.01 Da
• Isoelectric Point: 4.3466

>AT153789 WP_148759833.1 NZ_CP051206:19352-19585 [Dolichospermum flos-aquae CCAP 1403/13F]
INSMNELLNRITQIPGQCGGRPCIRGMRIRVSDILEMLAENVSVSEILEDFPDLEPEDIQACLVFAARRTDFVRLTA

Nucleotide

• Length: 234 bp

>AT153789 NZ_CP067093:c5512974-5512423 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCAATGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCTCTTTGCTCAACGATGAGAGTGCCAGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAAAGGCGGTTGGGCATATTTTATTCACCCGGGCGATGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGCGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCCATGGGAAGCCAGACGGTATTTGTTCTGGGCCACGCCACTTACTACCCCAGGCACGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGAGGCGCATAAAGCCTGCTGGATGCTACAGCGGCTTTCGTCT
CAGCCGCTGGGGCGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA