Detailed information of TA system TA153751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2803695..2804224
• Replicon: chromosome
• Accession: NZ_CP051191
• Organism: Staphylococcus aureus strain NGA102

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HF084_RS13950
• Protein ID: WP_000621175.1
• Coordinates: 2803862..2804224 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: HF084_RS13945
• Protein ID: WP_000948331.1
• Coordinates: 2803695..2803865 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF084_RS13915 (2798731)	2798731..2799291	+	561	WP_001092416.1	K(+)-transporting ATPase subunit C	-
HF084_RS13920 (2799500)	2799500..2799979	+	480	WP_001287090.1	hypothetical protein	-
HF084_RS13925 (2799972)	2799972..2801555	+	1584	WP_001294626.1	PH domain-containing protein	-
HF084_RS13930 (2801542)	2801542..2802033	+	492	WP_001205910.1	PH domain-containing protein	-
HF084_RS13935 (2802037)	2802037..2802396	+	360	WP_000581200.1	holo-ACP synthase	-
HF084_RS13940 (2802462)	2802462..2803610	+	1149	WP_001281151.1	alanine racemase	-
HF084_RS13945 (2803695)	2803695..2803865	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HF084_RS13950 (2803862)	2803862..2804224	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HF084_RS13955 (2804573)	2804573..2805574	+	1002	WP_061839378.1	PP2C family protein-serine/threonine phosphatase	-
HF084_RS13960 (2805693)	2805693..2806019	+	327	WP_001052491.1	anti-sigma factor antagonist	-
HF084_RS13965 (2806021)	2806021..2806500	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
HF084_RS13970 (2806475)	2806475..2807245	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T153751 WP_000621175.1 NZ_CP051191:2803862-2804224 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T153751 NZ_CP067071:1657683-1658123 [Rhizobium sp. AB2/73]
GTGACATTTCTGTTGGACGTCAATGTTCTGATCGCTTTATTCGATCCGAGCCATGTATCGCATGATATCGCCCATGAGTG
GTTTCACTCCCTAGGCGGCGACAGTTGGGCAACCTGCCCGTTAACGGAAAATGGCGTTGTTCGAATTCTCAGTCAGCCGA
GCTATCCCAACTCGCCCGGTCCACCGTCGACGGCCGCGCTTCTTGTTGCCCAGCTTCGCCGTTTGCCCGGTCACCAGTTC
TGGGCGGACGATATCAGTCTGCTGGACGATACTTTGGTGGATTTGACACGCATTCTGACTCCTGGACAGATCACCGACAC
CTATCTGTTGGCATTGGCGAAATTCCATAGAGGCCAACTGGCAACGCTCGACCGGCGCCTATCGACTTCAGCCGTTAAGG
ACGGCAAAGCCGCACTCCATATCATCAGCAGCAACGCGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT153751 WP_000948331.1 NZ_CP051191:2803695-2803865 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT153751 NZ_CP067071:1657459-1657686 [Rhizobium sp. AB2/73]
ATGCGAACAACTCTTGCAATTGACGACGACGTACTTGCCGCGGCAAAGGCGATGGCAGAGCAGCAGCACCGCAGCATCGG
TGATGTCATTTCCGACCTTGCTCGCCGATCGCTAAACCGTTCACAGCCGGCAAAGGAGCGGAATGGTATTCCGTTGCTCA
CGCCCAAGACAGGCAATCCCCCTGTTACGCTCGATCTTGTCAACAAACTTCGTGACGAGCTTCCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1