Detailed information of TA system TA153741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1262285..1262762
• Replicon: chromosome
• Accession: NZ_CP051191
• Organism: Staphylococcus aureus strain NGA102

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: HF084_RS06475
• Protein ID: WP_000052076.1
• Coordinates: 1262574..1262762 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: HF084_RS06470
• Protein ID: WP_001059690.1
• Coordinates: 1262285..1262545 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF084_RS06435 (1257680)	1257680..1258396	-	717	WP_000150021.1	type I 3-dehydroquinate dehydratase	-
HF084_RS06440 (1258544)	1258544..1258966	+	423	WP_000974447.1	organic hydroperoxide resistance protein	-
HF084_RS06445 (1259365)	1259365..1259859	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
HF084_RS06450 (1260013)	1260013..1260630	+	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
HF084_RS06455 (1260703)	1260703..1261284	-	582	WP_001140690.1	histidine phosphatase family protein	-
HF084_RS06460 (1261509)	1261509..1261745	+	237	WP_000368687.1	hypothetical protein	-
HF084_RS06465 (1261742)	1261742..1261945	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
HF084_RS06470 (1262285)	1262285..1262545	+	261	WP_001059690.1	hypothetical protein	Antitoxin
HF084_RS06475 (1262574)	1262574..1262762	+	189	WP_000052076.1	hypothetical protein	Toxin
HF084_RS06480 (1262950)	1262950..1263519	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
HF084_RS06485 (1263607)	1263607..1263891	+	285	WP_001004395.1	hypothetical protein	-
HF084_RS06490 (1263953)	1263953..1264171	+	219	WP_000005889.1	hypothetical protein	-
HF084_RS06495 (1264666)	1264666..1264866	-	201	WP_001059082.1	cold-shock protein	-
HF084_RS06500 (1265223)	1265223..1265909	-	687	WP_001641381.1	thermonuclease family protein	-
HF084_RS06505 (1266250)	1266250..1266759	-	510	WP_000824480.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-93)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T153741 WP_000052076.1 NZ_CP051191:1262574-1262762 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T153741 NZ_CP067066:c4799994-4799707 [Raoultella sp. XY-1]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGATCGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGGC
TTGTTCATGGCGTGATTGTTGTTCTTGTGGCGCATTCGATCCGATTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACGGCAAAGAGAGGAGTCGTTATGAGCACAGTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT153741 WP_001059690.1 NZ_CP051191:1262285-1262545 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT153741 NZ_CP067066:c4799720-4799415 [Raoultella sp. XY-1]
ATGAGCACAGTTAAATACAAGCGCGACAATCCGGCCGGGCTTAGCGCTCAGCGTGAAGACGAGCTCAGAGCGCTGGAGAA
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAATGCGATTTTAAGAGAAGCGATGATTCGCGAGCAGAATAAGAAATCGTAG

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56